I-SDS-PAGE Gel Electrophoresis System

Inkcazelo emfutshane:

I-Electrophoresis yindlela yaselabhoratri esebenzisa umsinga wombane ukwahlula i-DNA, i-RNA okanye iiproteni ezisekelwe kwiimpawu zazo zomzimba ezifana nobukhulu kunye nentlawulo. I-DYCZ-24DN yiseli encinci ye-electrophoresis engasetyenziselwa i-SDS-PAGE i-gel electrophoresis. I-SDS-PAGE, igama elipheleleyo yi-sodium dodecyl sulfate-polyacrylamide gel electrophoresis, esetyenziswa ngokuqhelekileyo njengendlela yokwahlula iiprotheni ezinobunzima be-molecular phakathi kwe-5 kunye ne-250 kDa. Bubuchule obusetyenziswa ngokubanzi kwi-biochemistry, i-molecular biology kunye ne-biotechnology ukwahlula iiproteni ngokusekelwe kubunzima bemolekyuli.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Inkcazo

Inkcazo yobuGcisa ye-DYCZ-24DN
Ubungakanani (LxWxH)	140×100×150mm
Ubungakanani beGel (LxW)	75×83mm
Ikama	Amaqula ali-10 kunye namaqula ali-15
Ukutyeba kweKamela	1.0mm kunye ne-1.5mm(Umgangatho）0.75mm (Ngokuzikhethela)
Inani leeSampulu	20-30
Buffer Volume	400 ml
Ubunzima	1.0kg
Inkcazo yobuGcisa ye-DYY-6C
Ubungakanani (LxWxH)	315 x 290x 128mm
Umbane ophumayo	6-600V
Imveliso yangoku	4-400mA
Amandla emveliso	240W
Isikhululo semveliso	Izibini ezi-4 ngokuhambelanayo
Ubunzima	5.0kg

Inkcazo

I-DYCZ–24DN yiseli ye-electrophoresis encinci emibini emi nkqo esetyenziselwa iprotein electrophoresis, eethe-ethe, elula kwaye kulula ukuyisebenzisa inkqubo. Yenziwe kwi-polycarbonate ephezulu kunye ne-electrode yeplatinam. Inkqubo iqulethe umzimba wetanki engundoqo (i-gel casting stand), isiciko esinezikhokelo, itanki yangaphandle (itanki ye-buffer) kunye nesixhobo sokuphosa ijeli. Izixhobo: ipleyiti yeglasi, ikama, ibhodi yeglasi etyebileyo (∮=5 mm) yokusebenzisa ijeli enye, isakhelo esikhethekileyo seweji. Ixhobisa i-10 kunye ne-15 imithombo ye-combs kunye ne-1.0mm kunye ne-1.5mm ubukhulu, kwaye ibonelela ngekama ozikhethelayo kunye nobukhulu be-0.75mm kunye nepleyiti yeglasi efakwe kwi-regula (0.75 mm) oyikhethayo. Isiseko sayo esicacileyo esingenamthungo kunye nesitofu esibunjiweyo sithintela ukuvuza kunye nokwaphuka. Inokugcina isisombululo se-buffer, isisombululo se-buffer esisisiseko simalunga ne-170 ml; kuphela i-170 ml yesisombululo se-buffer esinokugqibezela umfuniselo. Le nkqubo ikhuselekile kakhulu kubasebenzisi. Umthombo wayo wamandla uya kucinywa xa umsebenzisi evula isiciko. Uyilo olukhethekileyo lwesivalo luphepha ukwenza iimpazamo.

I-DYY-6C ngumbane olungiselelwe i-electrophoresis ukudala umbane wamandla ombane we-DNA / RNA ukuhlukana, i-PAGE electrophoresis kunye nokudluliselwa kwi-membrane. I-DYY-6C ixhasa imveliso ye-400V, 400mA, kunye ne-240W. I-LCD yayo ingabonisa i-voltage, yangoku, amandla kunye nexesha lexesha ngaxeshanye. Iyakwazi ukusebenza kwimeko eqhubekayo yombane, okanye kwimeko eqhubekayo yombane, kwaye iguqulelwe ngokuzenzekelayo ngokweeparitha ezibekwe kwangaphambili kwiimfuno ezahlukeneyo.

Isicelo

I-DYCZ-24DN kunye nombane we-DYY-6C yinkqubo ye-electrophoresis ye-SDS-PAGE okanye i-Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis yokwahlula iiprotheni kwaye isetyenziswa ngokubanzi kwi-forensics, i-genetics, i-biotechnology kunye nemimandla ye-molecular biology. Abaphandi bashwankathela ezi zicelo zilandelayo ze-SDS-PAGE:
1.Isetyenziselwa ukulinganisa ubunzima bemolekyuli yeemolekyuli.
2.Isetyenziselwa ukuqikelela ubungakanani beprotheni.
3.Isetyenziswa kwimephu yepeptide
4.Isetyenziselwa ukuthelekisa ukubunjwa kwepolypeptide yezakhiwo ezahlukeneyo.
5.Isetyenziselwa ukuqikelela ukucoceka kweeprotheni.
6.Isetyenziswa kwi-Western Blotting.
7.Isetyenziswa kuvavanyo lwe-HIV ukwahlula iiproteni ze-HIV.
8.Ukuhlalutya ubungakanani kunye nenani leepolypeptide subunits.

Uphawu

I-DYCZ-24DN yenziwe ngezinto ezibonakalayo ezikumgangatho ophezulu, kunye nenkangeleko ethambileyo, eyamkelwa ngokubanzi ngabathengi bethu. Ineempawu ezilandelayo:
• Yenziwe ngepolycarbonate ecacileyo ekumgangatho ophezulu, egqwesileyo kwaye ihlala ixesha elide, kulula ukuyibona;
• Ngokuphoswa kwejeli kwindawo yokuqala, ekwazi ukuphosa kunye nokuqhuba ijeli kwindawo enye, elula kwaye ilungele ukwenza iigels, kwaye ugcine ixesha lakho elixabisekileyo;
• Uyilo olukhethekileyo lwesakhelo se-wedge sinokulungisa igumbi le-gel ngokuqinileyo;
• Itanki ebunjiweyo yesithinteli inee-electrode zeplatinam ezisulungekileyo;
• Kulula kwaye kulula ukongeza iisampuli;
• Ukwazi ukusebenzisa ijeli enye okanye ezimbini ngexesha elinye;
• Gcina isisombululo se-buffer;
• Uyilo olukhethekileyo lwetanki luthintele isithinteli kunye nokuvuza kwejeli;
• I-electrodes esuswayo, kulula ukuyigcina kunye nokucoca;
• Cima ngokuzenzekelayo xa isiciko sivulwa;

I-DYY-6C njengombane wethu wokuthengisa oshushu unombane ozinzileyo kunye nangoku. Oku kulandelayo ziimpawu zayo ezizodwa:
• Ulawulo olukrelekrele lweprosesa yekhompyutha;
• Ukwazi ukulungelelanisa iiparamitha ngexesha langempela phantsi kwemeko yokusebenza;
• I-LCD yesikrini esikhulu ibonisa amandla ombane, angoku, amandla kunye nexesha lexesha ngaxeshanye.
• I-voltage, yangoku kunye namandla okulawula i-loop evaliweyo, ukuqonda ukulungiswa ngexesha lokusebenza.
• Ngomsebenzi wokubuyisela.
• Emva kokufikelela kwixesha elimiselweyo, linomsebenzi wokugcina umsinga omncinci.
• Ukhuseleko olugqibeleleyo kunye nomsebenzi wokulumkisa kwangethuba.
• Ngomsebenzi wokugcinwa kwememori.
• Umatshini omnye oneendawo zokubeka ezininzi, iziphumo ezine ezinxuseneyo.