Iseli ye-Electrophoresis ye-SDS-PAGE kunye ne-Western Blot
Inkcazo
| Ubungakanani (L×W×H) | 140×100×150mm |
| Ubungakanani beGel (L×W) | 75×83mm |
| Ikama | Amaqula ali-10 kunye namaqula ali-15 |
| Ukutyeba kweKamela | 1.0mm kunye 1.5mm (Eqhelekileyo) 0.75mm (Ngokuzikhethela) |
| Inani leeSampulu | 20-30 |
| Buffer Volume | 400ml |
| Ubunzima | 1kg |
Inkcazo
I-DYCZ-24DN i-electrophoresis cell cell (itanki / igumbi) ye-SDS-PAGE, i-Native PAGE njl njl.Le seli inokuphosa kwaye iqhube ijeli kwindawo enye.Ithambile kwaye ikhethekile kwaye kulula kwaye kulula ukulayisha iisampuli.Itanki yenziwe ngezinto eziphezulu zepolycarbonate, ehlala ixesha elide kwaye ibonakala.Le tanki ecacileyo yenza kube lula ukujonga ijeli xa usenza umfuniselo.I-DYCZ-24DN inee-electrodes ezisuswayo ezilula ukugcinwa.Ii-electrodes zenziwe ngeplatinam ecocekileyo (≥99.95%) eyi-electrolysis-corrosion kwaye imelana nobushushu obuphezulu.
Emva kwe-gel electrophoresis, ngokwemfuno yovavanyo, ngamanye amaxesha, umvavanyi kufuneka adlulisele i-gel kwinkxaso eqinile yokuhlalutya okuqhubekayo.Kubizwa ngokuba lilingo lokuvala, eliyindlela yokudlulisa iiproteni, i-DNA okanye i-RNA kumthwali.Yenziwa emva kwe-electrophoresis ye-gel, idlulisela iimolekyuli ukusuka kwi-gel ukuya kwi-membrane yokuqhawula.Emva kokucinywa, iiprotheyini ezigqithiselweyo, i-DNA okanye i-RNA zibonwa ngokucolwa kombala (umzekelo, ukungcoliswa kwesilivere yeeproteni), ukubonwa kwe-autoradiographic yeemolekyuli ezibhalwe ngeradiolabelled (olwenziwa phambi kweblot), okanye ukuleyibhelishwa okuthile kwezinye iiproteni okanye ii-nucleic acid.Le yokugqibela yenziwa ngee-antibodies okanye i-hybridization probes ezibophelela kuphela kwiimolekyuli ezithile zeblothi kwaye zine-enzyme edityaniswe kuzo.Emva kokuhlanjwa okufanelekileyo, lo msebenzi we-enzymatic (kwaye ke, iimolekyuli esizikhangelayo kwiblothi) zibonwa ngokufukama kunye ne-reactive eyiyo, inikezela nokuba yidiphozithi enemibala kwiblothi okanye i-reaction ye-chemiluminescent ebhaliswe ngefilimu yezithombe.
Kunikezelo lwamandla kule seli ethe nkqo yejeli ye-electrophoresis, sicebisa ukuba ibe nye yesixhobo sokulawula ixesha le-electrophoresis imodeli ye-DYY-6C.
Isicelo
I-SDS-PAGE, i-electrophoresis ye-Native PAGE kunye nokudlulisa i-molecule yeprotheni ukusuka kwijeli ukuya kwi-membrane.
Uphawu
Iseli ye-DYCZ-24DN encinci yegel electrophoresis encinci ye-SDS-PAGE, i-electrophoresis ye-Native PAGE inezi mpawu zilandelayo:
•Yenziwe ngepolycarbonate ecacileyo ekumgangatho ophezulu, egqwesileyo kwaye ihlala ixesha elide, kulula ukuyibona;
• Ngokuphoswa kwe-gel kwindawo yokuqala, ekwazi ukuphosa kunye nokuqhuba i-gel kwindawo enye, elula kwaye ilungele ukwenza iigels, kwaye ugcine ixesha lakho elixabisekileyo;
• Uyilo olukhethekileyo lwesakhelo se-wedge sinokulungisa igumbi le-gel ngokuqinileyo;
• Itanki ebunjiweyo yesithinteli inee-electrode zeplatinam ezisulungekileyo;
• Kulula kwaye kulula ukongeza iisampuli;
•Uyakwazi uku-rijeli enye okanye ezimbini ngexesha elinye;
• Gcina isisombululo se-buffer;
• Uyilo olukhethekileyo lwetanki luthintele isithinteli kunye nokuvuza kwejeli;
•I-electrodes esuswayo, kulula ukuyigcina kwaye icocekile;
• Cima ngokuzenzekelayo xa isiciko sivulwa;
Imodyuli ye-electrode, ebizwa ngokuba ngumzimba oxhasayo wokudluliselwa okanye ukudibanisa i-electrode yinxalenye ephambili yenkqubo yokuqhawula i-DYCZ-40D.Iquka iindawo zombala obomvu kunye nombala omnyama kunye ne-electrode ebomvu neyomnyama ukuze kuqinisekiswe ukuqhelaniswa okufanelekileyo kwejel ngexesha lokudluliselwa, kunye noyilo olusebenzayo olwenza lula ukufakwa kunye nokususwa kweekhasethi ze-gel holder ukusuka kumzimba oxhasayo wokudluliselwa (indibano ye-electrode).
