Amanyathelo okusebenza kwiSemi-dry Trans Blot Apparatus DYCP-40C

I-DYCP-40C i-semi-dry blotting system isetyenziswa kunye ne-electrophoresis power supply yokudlulisa iiproteni kwiijeli ze-polyacrylamide kwi-membrane efana ne-nitrocellulose membrane, inwebu yenayiloni kunye ne-PVDF inwebu.I-semi-dry blotting yenziwa nge-electrode yepleyiti yegraphite kuqwalaselo oluthe tye, isandwich ijeli kunye nenwebu phakathi kwamaphepha ephepha lokucoca elifakwe isithinteli elisebenza njengedama le-ion.Ngethuba lokudluliselwa kwe-electrophoretic, iimolekyuli ezihlawuliswa kakubi zifuduka ngaphandle kwejel kwaye ziqhubele kwi-electrode efanelekileyo, apho zifakwe kwi-membrane.I-electrodes yepleyiti, ehlulwe kuphela yi-gel kunye ne-stack paper stack, inika amandla aphezulu entsimi (V / cm) kwi-gel, eqhuba ukudluliselwa ngokukhawuleza, ngokukhawuleza.Umphezulu wokudluliselwa kwe-DYCP encinci - i-40C iseli ye-electrophoresis yi-150 × 150 (mm), ifanelekile ukuhambisa iigels eziqhelekileyo, kubandakanywa nezo zivela kwi-DYCZ-24DN kunye ne-DYCZ-24EN electrophoresis cell.

Masifunde ngakumbi malunga nokusebenza kwesi sixhobo se-semi-dry trans blot.

Izinto eziphathekayo, izixhobo zokusebenza kwe-DYCP-40C
Electrophoresis unikezelo lwamandla DYY-6C, semi-yomileyo trans blot apparatus DYCP-40C, isisombululo buffer, kunye nezikhongozeli isisombululo buffer.njl.

Amanyathelo okusebenza
1. Beka ijeli kunye neepleyiti zeglasi kwisisombululo sokudluliselwa kwebuffer

2. Ukulinganisa ubungakanani bejeli

3.Lungiselela iziqwenga ezi-3 zephepha lokucoca ngokobungakanani bejeli, kwaye ubungakanani bephepha lokucoca kufuneka libe likhulu kancinane kunobungakanani bejeli;Apha sisebenzisa iphepha lokucoca i-Whatman;

4.Beka amaqhekeza ama-3 ephepha lokucoca kwisisombululo se-buffer kancinane, kwaye uvumele iphepha lokucoca lintywiliselwe kwisithinteli ngokupheleleyo, kwaye uphephe ukuba namaqamza omoya;

5.Lungiselela kwaye usike i-nitrocellulose membrane ngokobungakanani bejel kunye nephepha lokucoca;ubukhulu be-nitrocellulose membrane kufuneka bube bukhulu kunobukhulu be-gel kunye nephepha lokucoca;

6.Faka i-nitrocellulose membrane kwisisombululo se-buffer;

7.Khupha amaqhekeza ama-3 ephepha lokucoca kwaye ulahle isisombululo esongezelelweyo sesithinteli kude kungabikho sisombululo sesithinteli siwisa kwinwebu;kwaye emva koko ubeke iphepha lokucoca phantsi kwe-DYCP-40C;

8.Thatha i-gel kwiiplate zeglasi, ucoce ngobumnene i-gel stacking, kwaye ubeke i-gel kwisisombululo se-buffer;

9.Beka i-gel kwiphepha lokucoca, qalisa ukusuka kwelinye icala le-gel ukuze ugweme ama-bubbles emoyeni;

10.Sebenzisa isixhobo esifanelekileyo ukususa amaqamza omoya phakathi kwejeli kunye nephepha lokucoca.

11.Gubungela i-nitrocellulose membrane kwi-gel, icala elibi ukuya kwijeli.Emva koko sebenzisa isixhobo esifanelekileyo sokususa amaqamza omoya phakathi kwenwebu kunye nejeli.Beka amaqhekeza ama-3 ephepha lokucoca kwi-membrane.Kusafuneka usebenzise isixhobo esifanelekileyo ukususa amaqamza omoya phakathi kwephepha lokucoca kunye nenwebu.

12.Gubungela isiciko, kwaye usethe i-electrophoresis esebenzayo iparameters, i-80mA yangoku rhoqo;

13.I-electrophoresis yenziwe.Sifumana umphumo njengokulandelayo;

I-Beijing Liuyi Biotechnology Co., Ltd ikhethekileyo kwimveliso ye-electrophoresis iminyaka engaphezu kwama-50.Siyinkampani eqinisekisiweyo ye-ISO9001 kunye ne-ISO13485 kwaye sisebenza ngokukhethekileyo ekwenzeni iitanki ze-electrophoresis, izixhobo zombane, i-UV transilluminator, kunye namaxwebhu ejeli kunye nenkqubo yohlalutyo, okwangoku, sibonelela ngenkonzo ye-OEM kubathengi bethu, kunye nenkonzo ye-ODM.

Ngoku sikhangela amaqabane, zombini i-OEM kunye nabasasazi bamkelwe.

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