Amanyathelo okusebenza kwiSemi-dry Trans Blot Apparatus DYCP-40C

I-DYCP-40C i-semi-dry blotting system isetyenziswa kunye ne-electrophoresis power supply yokudlulisela iiproteni kwiigels ze-polyacrylamide kwi-membrane efana ne-nitrocellulose membrane, inwebu ye-nayiloni kunye ne-PVDF inwebu. I-semi-dry blotting yenziwa nge-electrode yepleyiti yegraphite kuqwalaselo oluthe tye, isandwich ijeli kunye nenwebu phakathi kwamaphepha ephepha lokucoca elifakwe isithinteli elisebenza njengedama le-ion. Ngethuba lokudluliselwa kwe-electrophoretic, iimolekyuli ezihlawuliswa kakubi zifuduka ngaphandle kwejel kwaye ziqhubele kwi-electrode efanelekileyo, apho zifakwe kwi-membrane. I-electrodes yepleyiti, ehlulwe kuphela yi-gel kunye ne-stack paper stack, inika amandla aphezulu entsimi (V / cm) kwi-gel, eqhuba ukudluliselwa ngokukhawuleza, ngokukhawuleza. Umphezulu wokudluliselwa kwe-DYCP encinci - i-40C ye-electrophoresis cell yi-150 × 150 (mm), ifanelekile ukuhambisa iigels eziqhelekileyo, kubandakanywa nezo zivela kwi-DYCZ-24DN kunye ne-DYCZ-24EN electrophoresis cell.

Masifunde ngakumbi malunga nokusebenza kwesi sixhobo se-semi-dry trans blot.

Izinto eziphathekayo, izixhobo zokusebenza kwe-DYCP-40C
Umbane we-Electrophoresis i-DYY-6C, i-semi-dry trans blot apparatus DYCP-40C, isisombululo se-buffer, kunye nezikhongozeli zesisombululo se-buffer. njl.

Amanyathelo okusebenza
1. Beka ijeli kunye neepleyiti zeglasi kwisisombululo sokudluliselwa kwebuffer

2. Ukulinganisa ubungakanani bejeli

3.Lungiselela iziqwenga ezi-3 zephepha lokucoca ngokobungakanani bejeli, kwaye ubungakanani bephepha lokucoca kufuneka libe likhulu kancinane kunobungakanani bejeli; Apha sisebenzisa iphepha lokucoca i-Whatman;

4.Beka amaqhekeza ama-3 ephepha lokucoca kwisisombululo se-buffer kancinane, kwaye uvumele iphepha lokucoca lintywiliselwe kwisithinteli ngokupheleleyo, kwaye uphephe ukuba namaqamza omoya;

5.Lungiselela kwaye usike i-nitrocellulose membrane ngokobungakanani bejel kunye nephepha lokucoca; ubukhulu be-nitrocellulose membrane kufuneka bube bukhulu kunobukhulu be-gel kunye nephepha lokucoca;

6.Faka i-nitrocellulose membrane kwisisombululo se-buffer;

7.Khupha amaqhekeza ama-3 ephepha lokucoca kwaye ulahle isisombululo esongezelelweyo sesithinteli kude kungabikho sisombululo sesithinteli siwisa kwinwebu; kwaye emva koko ubeke iphepha lokucoca phantsi kwe-DYCP-40C;

8.Thatha i-gel kwiiplate zeglasi, ucoce ngobumnene i-gel stacking, kwaye ubeke i-gel kwisisombululo se-buffer;

9.Beka i-gel kwiphepha lokucoca, qalisa ukusuka kwelinye icala le-gel ukuze ugweme ama-bubbles emoyeni;

10.Sebenzisa isixhobo esifanelekileyo ukususa amaqamza omoya phakathi kwejeli kunye nephepha lokucoca.

11.Gubungela i-nitrocellulose membrane kwi-gel, icala elibi ukuya kwijeli. Emva koko sebenzisa isixhobo esifanelekileyo sokususa amaqamza omoya phakathi kwenwebu kunye nejeli. Beka amaqhekeza ama-3 ephepha lokucoca kwi-membrane. Kusafuneka usebenzise isixhobo esifanelekileyo ukususa amaqamza omoya phakathi kwephepha lokucoca kunye nenwebu.

12.Gubungela isiciko, kwaye usethe i-electrophoresis esebenzayo iparameters, i-80mA yangoku rhoqo;

13.I-electrophoresis yenziwe. Sifumana umphumo njengokulandelayo;

I-Beijing Liuyi Biotechnology Co., Ltd ikhethekileyo kwimveliso ye-electrophoresis iminyaka engaphezu kwama-50. Siyinkampani eqinisekisiweyo ye-ISO9001 kunye ne-ISO13485 kwaye sisebenza ngokukhethekileyo ekuveliseni iitanki ze-electrophoresis, izixhobo zombane, i-UV transilluminator, kunye namaxwebhu ejeli kunye nenkqubo yohlalutyo, okwangoku, sibonelela ngenkonzo ye-OEM kubathengi bethu, kunye nenkonzo ye-ODM.

Ngoku sikhangela amaqabane, zombini i-OEM kunye nabasasazi bamkelwe.

Ukuba unayo nayiphi na isicwangciso sokuthenga iimveliso zethu, nceda ungalibazisi ukudibana nathi. Ungasithumela umyalezo nge-imeyile[i-imeyile ekhuselweyo]okanye[i-imeyile ekhuselweyo], okanye nceda usitsalele umnxeba +86 15810650221 okanye wongeze iWhatsapp +86 15810650221, okanye Wechat: 15810650221.