Ukucinywa kweeProtheyini
Iprotein blotting, ekwabizwa ngokuba yi-western blotting, ukuhanjiswa kweeprotheni kwiinkxaso ze-membrane yesigaba esiqinileyo, bubuchule obunamandla kwaye obudumileyo bokubonwa kunye nokuchongwa kweeprotheni.
Ngokubanzi, iprotein yokucima ukuhamba komsebenzi ibandakanya ukhetho lwendlela efanelekileyo, izixhobo, inwebu, ibuffer, kunye neemeko zodluliselo.Emva kokuba iiprotheyini zingenakunyakaziswa kwi-membrane, ziyafumaneka ukuze zibonwe, zibonwe, kwaye zihlalutywe.
I-Liuyi Biotechnology ibonelela ngobungakanani obahlukeneyo benkqubo ye-electrophoresis ethe nkqo enokufikelela kwiimfuno zakho zovavanyo.Ezinye zeeseli ze-electrophoresis zikaLiuyi ezithe nkqo (iitanki/amagumbi) ziyahambelana nendibano yethu ye-electrode yokuhambisa imolekyuli yeproteni ukusuka kwijeli ukuya kwinwebu kumfuniselo wokucinywa wentshona.Iinkqubo ye-electrophoresis ethe nkqoimodeliI-DYCZ-24DN, I-DYCZ-25DkwayeI-DYCZ-25Eziyahambelana nemodeli yenkqubo yokudlulisa itankiI-DYCZ-40D, I-DYCZ-40GkwayeI-DYCZ-40F.Umahluko omkhulu malunga nala matanki bubungakanani betanki yomthamo we-buffer, kunye nobungakanani bejeli.Iinkqubo ye-electrophoresis ethe nkqoimodeliI-DYCZ-25Dinoyilo lokuphelisa ubushushu ukwenzela ukupholisa ngcono ngexesha lokuqhuba iijeli.
Indlela yokubhula
Kukho iindlela ezahlukeneyo zokudluliselwa, kodwa indlela ethandwa kakhulu yi-electrophoretic transfer, kuba ikhawuleza kwaye isebenza ngakumbi kunezinye iindlela.Kukho iindlela ezintathu ezinokuthi zidlulise iprotheni ukusuka kwi-SDS-PAGE okanye i-gel kwi-membrane: ukuhanjiswa kwetanki, ukuhanjiswa kwe-semi-dry kunye nokudluliselwa okomileyo.Beijing liwuyiI-Biotechnologyuyeiimveliso zeukudluliselwa kwetanki, ukudluliselwa kwe-semi-dry, kwaye usazise iinkcukacha ezingakumbi malungaukuhanjiswa kwetankikwayeugqithiso oluphakathi.
Iinkqubo zokudluliselwa kwetanki - iigels kunye neembrane zifakwe kwi-buffer yokudlulisa kwiitanki;ezi nkqubo ziluncedo kuninzi lomsebenzi wesiqhelo weprotheyini, kugqithiso lweprotheyini esebenzayo kunye nobungakanani, kunye nokudluliselwa kweeproteni zabo bonke ubukhulu.Iisistim zokuhanjiswa kwetanki zibonelela ngoguquko olukhulu ekukhetheni useto lombane, amaxesha okucinywa, kunye neenketho zokupholisa.
Iinkqubo ze-semi-dry - i-gel kunye ne-membrane zixutywe phakathi kwamaphepha okucoca i-buffer-saturated adibene ngokuthe ngqo kunye nee-electrode zeplate;ezi nkqubo ziqhelekile ukusetwa kunezixokelelwano zetanki kwaye ziluncedo xa ugqithiso oluphezulu luyimfuneko kwaye amaxesha ogqithiselo awandisiweyo engafuneki, okanye xa iinkqubo ze-buffer ezingapheliyo zisetyenziswa.Iinketho zokupholisa ezisebenzayo zikhawulelwe ngokuvalwa kwe-semi-dry blotting.
I-DYCP-40C
eBeijingI-Liuyi Biotechnology inokubonelela ngeenkqubo zokudlulisa itanki kunye neenkqubo zokomisa okomileyo ozikhethelayo.ImodeliI-DYCZ-40D, I-DYCZ-40F,kwayeI-DYCZ-40Gziinkqubo zethu zokuhambisa itanki;imodeliI-DYCP-40CkwayeI-DYCP-40Eziinkqubo ezomileyo.
I-brand ye-Liuyi ineminyaka engaphezu kwama-50 yembali e-China kwaye inkampani inokubonelela ngeemveliso ezizinzileyo kunye neziphezulu emhlabeni jikelele.
Ukuze ufumane inkcazelo engakumbi ngathi, nceda uqhagamshelane nathi nge-imeyile[i-imeyile ekhuselweyo], iintengiso01@ly.com.cn
Ixesha lokuposa: Apr-08-2022