Agawavukagel electrophoresis
I-Agarose gel electrophoresis yindlela ye-gel electrophoresis esetyenziswa kwi-biochemistry, i-biology ye-molecular, i-genetics, kunye ne-chemistry yeklinikhi ukwahlula inani elixubekileyo le-macromolecules njenge-DNA okanyeI-RNA.Oku kuphunyezwa ngokuhambisa iimolekyuli ze-nucleic acid ezihlawuliswa kakubi ngokusebenzisa i-agarose matrix ene-agarose matrix.i-electrophoresis.
Iipropati zejeli ye-agarose
Ijeli ye-Agarose yi-matrix ene-dimensional-dimensional ye-matrix ye-helical agarose kwii-bundle ezi-supercoiled ezidityanisiweyo zibe yi-dimensional-dimensional structures kunye nemijelo kunye ne-pores apho i-biomolecules inokudlula khona. ngokufudumeza ukubuyisela kwimeko yolwelo.Iqondo lokushisa elinyibilikayo lihluke kwiqondo lokushisa le-gelling, kuxhomekeke kwimithombo, ijeli ye-agarose inokushisa kwe-gelling ye-35.-42 °C kunye nobushushu obunyibilikayo obungama-85-95 °C.I-agaroses encibilikayo ephantsi kunye ne-low-gelling agaroses eyenziwe ngokuguqulwa kweekhemikhali nazo ziyafumaneka.
Izinto eziluncedo kunye nokungonakali kwe-agarose gel electrophoresis
Eyona nzuzo iphambili ye-agarose gel electrophoresis ubuchule kukuba inokucutshungulwa ngokulula kwaye imolekyuli ye-DNA esetyenziswa njengesampulu inokubuyiselwa ngaphandle kokwenzakala kuyo ekupheleni kwenkqubo.Ngenxa yokuba ijeli ye-agarose ayifuni't iqulethe i-sulfate kunye neqela le-carboxyl, ayiyi kubangela i-electroosmosis ye-agar.Ngesakhiwo esifanayo kunye nobukhulu obukhulu bepore, ijeli ye-agarose ilungileukwahlula iimolekyuli ezinkulu zeDNA, ezifana i-enzyme enzima, i-nucleic acid kunye nentsholongwane.Ijeli yeAgarose ayifuni't ukufunxa ukukhanya kwe-UV, ngoko inokungcoliswa ngokuthe ngqo okanye emva kokuba i-gelling ibe yifilim, iphinda ibuyele umva nge-thermal.
Kukwakho nezinye izinto ezingeloncedo ngobuchule be-agarose gel electrophoresis.Ijeli ye-Agarose iqhekezwe ngokulula, ngoko ke ubuninzi abukwazi ukuba ngaphantsi kakhulu, kuya kubiza i-agarose ngaphezulu.Ijeli ye-agarose kunzima ukuyigcina kuba kulula ukosulelwa ziintsholongwane, kwaye kufuneka ilungiswe kanye phambi kokuba isetyenziswe.Xa kuthelekiswa ne-PAGE electrophoresis ubuchule, ukusebenza kakuhle kwe-molecular sieve kuncinci, kwaye indawo ye-electrophoresis incinci.
Ukuthe tyeelectrophoresisinkqubo yejeli yeagarose
Ngaphandle kwe-agarose kunye ne-buffer, uvavanyo lwe-agarose gel electrophoresis lufuna inkqubo ye-electrophoresis ethe tye kunye ne-low-.i-voltage electrophoresis amandla ombane.I-Beijing Liuyi Biotechnology inokubonelela ngenkqubo ye-electrophoresis ethe tye kunye unikezelo lwamandla e-electrophoresis, njengemodeliI-DYCP-31BN, I-DYCP-31CN ngenxainkqubo ye-electrophoresis ethe tyaba kwayeDYY-6C ukubonelela ngombane.
Kuxhomekeke kwiisayizi ezahlukeneyo zejeliifunwa yi i-agrose gel electrophoresis experiment, ubukhulu obuhlukeneyo benkqubo ye-electrophoresis ethe tye inokunikezelwa yiBeijing Liuyi Biotechnology.Imodeli yeDYCP-31 uthotho,snjlI-DYCP-31BN, I-DYCP-31CN, I-DYCP-31DN, I-DYCP-31E, I-DYCP-32B, kwayeI-DYCP-32Czineesayizi ezahlukeneyo zetreyi yejeli, ukusuka ekujuleni ubungakanani bejeli encinci ukuya kwijeli yobungakanani obukhulu, banokuhlangabezana novavanyo lwakho.iimfuno.
I-brand ye-Liuyi ineminyaka engaphezu kwama-50 yembali e-China kwaye inkampani inokubonelela ngeemveliso ezizinzileyo kunye neziphezulu emhlabeni jikelele.
Ukuze ufumane inkcazelo engakumbi ngathi, nceda uqhagamshelane nathi nge-imeyile[i-imeyile ekhuselweyo], iintengiso01@ly.com.cn.
Ixesha lokuposa: Apr-16-2022