I-Gel electrophoresis yenye yeendlela eziphambili ezisetyenziswa kwibhayoloji yemolekyuli kuhlalutyo lweDNA.Le ndlela ibandakanya ukufuduka kwamaqhekeza e-DNA ngokusebenzisa ijeli, apho yahlulwe ngokusekelwe kubukhulu okanye imilo.Nangona kunjalo, ngaba ukhe wadibana naziphi na iimpazamo ngexesha lovavanyo lwakho lwe-electrophoresis, njengeebhendi eziqatyiweyo kwijeli ye-agarose, okanye akukho bands kwijeli?Yintoni enokuba ngunobangela wezi mpazamo?
Iingcali zethu ziye zashwankathela izibini zokusombulula ingxaki apha kwireferensi yakho.
1. Iibhanti eziqatyiweyo kwijeli yeagarose
●I-DNA yathotywa.Kulumkele ukungcoliseka kwe-nuclease.
● Isithinteli se-electrophoresis asikho ntsha.Emva kokusetyenziswa ngokuphindaphindiweyo kwe-electrophoresis buffer, amandla e-ionic ayancipha, kwaye ixabiso layo le-pH liyenyuka, ngoko umthamo we-buffer uyancipha, ochaphazela umphumo we-electrophoresis.Kucetyiswa ukuba utshintshe isithinteli se-electrophoresis rhoqo.
● Iimeko ezingafanelekanga ze-electrophoresis zasetyenziswa.Ungavumeli i-voltage ukuba idlule i-20 V / cm, kwaye ugcine izinga lokushisa <30 ° C ngexesha le-electrophoresis.Kwi-DNA strand electrophoresis enkulu, iqondo lobushushu kufuneka libe ngu-<15° C. Jonga i-electrophoresis buffer inomthamo owaneleyo we-buffer.
● I-DNA eninzi kakhulu yafakwa kwijeli.Nciphisa inani le-DNA.
● Ityuwa eninzi kwiDNA.Sebenzisa i-ethanol precipitation ukususa iityuwa ezingaphezulu.
● I-DNA yayineprotini.Sebenzisa i-phenol extractions ukususa iiprotheyini eziphambili.
● I-DNA yatshintshwa.Musa ukufudumala phambi kwe-electrophoresis.Nciphisa i-DNA kwi-buffer nge-20 mM NaCl.
2. I-Anomalies DNA band migration
● Ukuhlaziywa kwendawo ye-COS yeqhekeza le-λHind III.Fudumeza i-DNA imizuzu emi-5 ngaphantsi kwe-65° C phambi kwe-electrophoresis, uze uyipholise kwiyunithi yomkhenkce imizuzu emi-5.
● Iimeko ezingafanelekanga ze-electrophoresis zasetyenziswa.Ungavumeli i-voltage ukuba idlule i-20 V / cm, kwaye ugcine izinga lokushisa <30 ° C ngexesha le-electrophoresis.Jonga i-electrophoresis buffer inomthamo owaneleyo we-buffer.
● I-DNA yatshintshwa.Musa ukufudumala phambi kwe-electrophoresis.Nciphisa i-DNA kwi-buffer nge-20 mM NaCl.
3. Ukufa isiqaqa okanye akukho bhanti zeDNA kwijeli yeagarose
● Bekukho umyinge onganelanga okanye ukugxilwa kwe-DNA efakwe kwijeli.Yandisa inani le-DNA.I-Polyacrylamide gel electrophoresis inovakalelo kancinci kune-agarose electrophoresis, kwaye ukulayishwa kwesampulu kunokwehliswa ngokufanelekileyo.
● IDNA yonakala.Kulumkele ukungcoliseka kwe-nuclease.
● I-DNA yafakwa kwijeli ye-electrophore.I-Electrophorese i-gel ixesha elincinci, sebenzisa i-voltage ephantsi, okanye usebenzise i-gel ephezulu yeepesenti.
● Umthombo wokukhanya we-W ongafanelekanga wasetyenziselwa ukubonwa kwe-ethidium bromide-stained DNA.Sebenzisa ubude obufutshane (254 nm) W ukukhanya ngovakalelo olukhulu.
4. Iibhanti zeDNA azikho
●Ubungakanani obuncinci be-DNA benziwe nge-electrophoresed ngaphandle kwejeli.I-Electrophorese i-gel ixesha elincinci, sebenzisa i-voltage ephantsi, okanye usebenzise i-gel ephezulu yeepesenti.
● Kunzima ukwahlula amaqela e-DNA eemolekyuli ezifanayo.Yandisa ixesha le-electrophoresis, kwaye ujonge ukugxilayejeli ukuqinisekisa ukuba ijeli echanekileyo ngokwepesenti ekufuneka isetyenziswe.
● I-DNA yatshintshwa.Musa ukufudumala phambi kwe-electrophoresis.Nciphisa i-DNA kwi-buffer nge-20 mM NaCl.
● Imisonto ye-DNA mikhulu, kwaye ijeli ye-electrophoresis eqhelekileyo ayifanelekanga.Hlalutya kwi-pulse gel electrophoresis.Yeyiphi eminye imiba okhe waba nayo nge-agarose gel electrophoresis?Siza kuphanda ngakumbi kwizikhokelo kwixesha elizayo.
I-Beijing Liuyi biotechnology Co., Ltd (i-Liuyi Biotech) yinkampani ekhethekileyo egxile kwiimveliso ezinxulumene ne-electrophoresis e-China.Ibali layo liqala ngo-1970 xa iChina yayingekangeni kuhlaziyo kunye nexesha lokuvula.Ngokuhamba kweminyaka, i-Liuyi Bitotech ine-brand yayo, eyaziwa ngokuba yi-Liuyi Brand kwimarike yasekhaya yeemveliso ze-electrophoresis.
I-brand ye-Liuyi inembali engaphezulu kweminyaka engama-50 e-China kwaye inkampani inokubonelela ngeemveliso ezizinzileyo nezikumgangatho ophezulu kwihlabathi jikelele.Ukutyhubela iminyaka yophuhliso, ilufanele ukhetho lwakho!
Iiseli ze-electrophoresis ezinqamlekileyo (iitanki / amagumbi) e-Liuyi Biotech zisemgangathweni ophezulu kunye nokubonakala kakuhle.Ngobukhulu obahlukeneyo beetreyi zejeli, banokuhlangabezana neemfuno zakho zovavanyo olwahlukileyo.Sineqela lethu lobugcisa kunye nefektri.Ukusuka kuyilo ukuya ekwenziweni, izinto eziluhlaza ukuya kwiindawo eziphambili, sinokuyilawula yonke inkqubo.Uluhlu lwe-DYCP 31 lwenzelwe i-DNA electrophoresis, eziyimodeliI-DYCP-31BN, I-DYCP-31CN,I-DYCP-31DN, kwayeI-DYCP-31E.Ukwahluka phakathi kwabo kubukhulu bejeli kunye nexabiso.Sinikezela ngeesayizi ezipheleleyo zeemveliso kubathengi bethu.ImodeliI-DYCP-32Cingenza eyona gel inkulu 250mm * 250mm.
Okwangoku, sincoma umbane wethu we-electrophoresisDYY-6C,I-DYY-6DkwayeDYY-10Ckwiiseli zethu ze-electrophoresis (itanki / amagumbi) i-DYCP-31 kunye ne-32 series.
Ukuba ufuna ulwazi oluthe kratya malunga neemveliso, nceda undwendwele le webhusayithi ukuze ufumane okungakumbi, kwaye wamkelekile ukuba uqhagamshelane nathi nge-imeyile ukuze usazise into oyifunayo, kwaye ubone ukuba sinokukubonelela ngezisombululo.
Ukuze ufumane inkcazelo engakumbi ngathi, nceda uqhagamshelane nathi nge-imeyile[i-imeyile ekhuselweyo], [i-imeyile ekhuselweyo].
Ixesha lokuposa: May-09-2022