IPulsed Field Gel Electrophoresis CHEF Mapper A4

Inkcazelo emfutshane:

I-CHEF Mapper A4 ifanelekile ukufumanisa nokwahlula iimolekyuli zeDNA ukusuka kwi-100 bp ukuya kwi-10 Mb.Iquka iyunithi yokulawula, igumbi le-electrophoresis, iyunithi yokupholisa, ipompo yokujikeleza, kunye nezixhobo.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iinkcukacha

Umzekelo	UMPHEPHE Mapper A4
I-Voltage Gradient	0.5V/cm ukuya ku-9.6V/cm, inyuswe ngo-0.1V/cm
Ubuninzi bangoku	0.5A
Ubuninzi bombane	350V
I-Angle yePulse	0-360 °
Ixesha leGradiyenti	Umgca
Ukutshintsha Ixesha	50ms ukuya kwi-18h
Elona xesha liphezulu lokubaleka	999h
Inani lee-Electrodes	24, ilawulwa ngokuzimeleyo
Uluhlu lobushushu	0℃ ukuya ku-50℃, imposiso yobhaqo <±0.5℃

Inkcazo

I-pulsed field gel electrophoresis (PFGE) yahlula iimolekyuli ze-DNA ngokutshintshanisa intsimi yombane phakathi kwee-electrode pairs ezahlukeneyo zendawo, ezibangela iimolekyuli ze-DNA, ezinokuthi zibe zizigidi zezibini ezisisiseko ezide zilungelelanise kwaye zifuduke nge-agarose gel pores ngezantya ezahlukeneyo.Ifikelela kwisisombululo esiphezulu phakathi kolu luhlu kwaye isetyenziswa ikakhulu kwi-biology yokwenziwa;ukuchongwa kwemigca yebhayoloji kunye ne-microbial;uphando kwi-epidemiology yeemolekyuli;izifundo zamaqhekeza amakhulu eplasmid;ukufumaneka kwendawo yemfuza yezifo;imephu ebonakalayo yezakhi zofuzo, uhlalutyo lwe-RFLP, kunye ne-DNA fingerprinting;uphando lokufa kweeseli olucwangcisiweyo;izifundo ngomonakalo kunye nokulungiswa kwe-DNA;ukuhlukaniswa kunye nohlalutyo lwe-DNA ye-genomic;ukuhlukana kwe-chromosomal DNA;ukwakhiwa, ukuchongwa, kunye nohlalutyo lwamaqhekeza amakhulu amathala eencwadi e-genomic;kunye nophando lwe-transgenic.t ugxininiso olusezantsi njenge-0.5 ng/µL (dsDNA).

Isicelo

Ifanelekile ukufumanisa kunye nokwahlula iamolekyu ze-DNA ukusuka kwi-100bp ukuya kwi-10Mb ngobukhulu, ukufezekisa isisombululo esiphezulu ngaphakathi kolu luhlu.

Uphawu

• ITekhnoloji ePhakamileyo: Idibanisa i-CHEF kunye ne-PACE pulsed-field technologies ukuze kuphunyezwe iziphumo ezizezona zilungileyo ngemizila ethe tye, engagobiyo.

• Ulawulo oluZimeleyo: Iimpawu ze-electrode zeplatinam ezizimeleyo ezingama-24 (i-0.5mm ubukhulu), kunye ne-electrode nganye enokutshintshwa ngokuzimeleyo.

• Umsebenzi wokubala ngokuzenzekela: Udibanisa izinto ezininzi eziguquguqukayo eziphambili ezifana ne-voltage gradient, ubushushu, i-engile yokutshintsha, ixesha lokuqala, ixesha lokuphela, ixesha lokutshintsha langoku, ixesha lokubaleka lilonke, i-voltage, kunye ne-current kubalo oluzenzekelayo, ukunceda abasebenzisi ukuba bafezekise iimeko zovavanyo ezigqwesileyo.

• I-Algorithm ekhethekileyo: Isebenzisa i-algorithm yokulawula i-pulse ekhethekileyo kwimiphumo engcono yokuhlukana, ukwahlula ngokulula phakathi kwe-DNA yomgca kunye nesetyhula, kunye nokwahlula okuphuculweyo kwe-DNA enkulu yesetyhula.

• Ukuzenzekela: Rekhoda ngokuzenzekelayo kwaye iqalise kwakhona i-electrophoresis ukuba inkqubo iphazamisekile ngenxa yokusilela kombane.

Umsebenzisi-oLungisiweyo: Ivumela abasebenzisi ukuseta iimeko zabo.

• Ukuba bhetyebhetye: Isixokelelwano sinokukhetha ukuthambeka kwamandla ombane athile kunye namaxesha okutshintsha kuluhlu oluthile lobungakanani beDNA.

• Isikrini esikhulu: Ixhotyiswe nge-7-intshi ye-LCD yesikrini ukuze isebenze lula, ebonisa ulawulo olulodwa lwesofthiwe yokusetyenziswa okulula kunye nokulula.

• Ukubonwa koBubushushu: Iiprobe ezimbini zobushushu zibhaqa ngokuthe ngqo ubushushu be-buffer kunye nomda wempazamo ongaphantsi kwe ±0.5℃.

• Inkqubo yokujikeleza: Iza nenkqubo yokujikeleza kwe-buffer elawula ngokuchanekileyo kwaye ibeke esweni ubushushu besisombululo se-buffer, iqinisekisa ubushushu obuqhubekayo kunye ne-ionic balance ngexesha le-electrophoresis.

• Ukhuseleko oluPhezulu: Kubandakanya isigqubuthelo sokhuseleko se-acrylic esiselubala esiwacima umbane ngokuzenzekelayo xa siphakanyisiwe, kunye nemisebenzi yokhuseleko olugqithisileyo kunye nokungabikho komthwalo.

• Umgangatho ohlengahlengiswayo: Itanki ye-electrophoresis kunye nejeli ye-caster ibonakalisa iinyawo ezilungelelanisiweyo zokulinganisa.

• I-Mold Design: I-tank ye-electrophoresis yenziwe ngesakhiwo esidibeneyo sokubumba ngaphandle kokudibanisa;i-electrode rack ixhotyiswe nge-electrode ye-platinum eyi-0.5mm, iqinisekisa ukuqina kunye neziphumo zovavanyo ezizinzile.

FAQ

Umbuzo: Yintoni iPulsed Field Gel Electrophoresis?

A: I-Pulsed Field Gel Electrophoresis yindlela esetyenziselwa ukwahlula iimolekyuli ezinkulu zeDNA ngokusekelwe kubukhulu bazo.Kubandakanya ukutshintsha indlela yombane kwi-matrix yejeli ukwenzela ukuba ukwahlulwa kwamaqhekeza e-DNA emakhulu kakhulu ukuba angasonjululwa yi-agarose gel electrophoresis.

Umbuzo: Zeziphi izicelo zePulsed Field Gel Electrophoresis?

A: I-Pulsed Field Gel Electrophoresis isetyenziswa ngokubanzi kwibhayoloji yemolekyuli kunye nemfuzo ye:

Imephu yeemolekyuli ezinkulu zeDNA, ezinjengeechromosomes kunye neeplasmids.

• Ukumisela ubungakanani begenome.

• Ukufunda iiyantlukwano zemfuza kunye nobudlelwane bendaleko.

• I-epidemiology yeemolekyuli, ngakumbi ukulandelela ukuqhambuka kwezifo ezosulelayo.

• Uhlalutyo lomonakalo kunye nokulungiswa kwe-DNA.

• Ukumisela ubukho bemfuza ethile okanye ulandelelwano lweDNA.

Umbuzo: Isebenza njani iPulsed Field Gel Electrophoresis?

A: I-Pulsed Field Gel Electrophoresis isebenza ngokubeka iimolekyuli ze-DNA kwindawo yombane epholileyo etshintshana kwicala.Oku kuvumela iimolekyuli ezinkulu ze-DNA ukuba zilungelelanise kwakhona phakathi kwee-pulses, zivumela ukuhamba kwazo nge-matrix yejeli.Iimolekyuli ezincinci ze-DNA zihamba ngokukhawuleza kwijeli, ngelixa ezinkulu zihamba kancinci, zivumela ukuhlukana kwazo ngokusekelwe kubukhulu.

Umbuzo: Uthini umgaqo emva kwePulsed Field Gel Electrophoresis?

A: I-Pulsed Field Gel Electrophoresis iyahlula i-molecule ye-DNA ngokusekelwe kubukhulu bayo ngokulawula ubude kunye nolwalathiso lwee-pulses zentsimi zombane.Intsimi etshintshanayo ibangela ukuba iimolekyuli ezinkulu ze-DNA ziqhubeke zilungelelanisa, zikhokelela ekufudukeni kwazo nge-matrix ye-gel kunye nokwahlula ngokobukhulu.

Umbuzo: Ziziphi iingenelo zePulsed Field Gel Electrophoresis?

A: Isisombululo esiphezulu sokwahlula iimolekyuli ezinkulu ze-DNA ukuya kwii-pairs zesiseko sezigidi ezininzi.Ukwazi ukusombulula kunye nokwahlula iziqwenga ze-DNA zobukhulu obufanayo.I-Versatility kwisicelo, ukusuka kwi-microbial typing ukuya kwi-molecular genetics kunye ne-genomics.Indlela esekelwe kwizifundo ze-epidemiological kunye nemephu yezofuzo.

Umbuzo: Ziziphi izixhobo ezifunekayo kwiPulsed Field Gel Electrophoresis?

A: I-Pulsed Field Gel Electrophoresis ifuna i-electrophoresis apparatus ene-electrode ezikhethekileyo zokuvelisa imimandla yepulsed.Ijeli yeAgarose matrix enogxininiso olufanelekileyo kunye nesithinteli.Ukunikezelwa kwamandla okukwazi ukuvelisa i-voltage high-voltage pulses.Inkqubo yokupholisa ukukhupha ukushisa okwenziwa ngexesha le-electrophoresis, kunye nepompo yokujikeleza.