IPulsed Field Gel Electrophoresis CHEF Mapper A1
Iinkcukacha
| Umzekelo | I-CHEF Mapper A1 |
| I-Voltage Gradient | 0.5V/cm ukuya ku-9.6V/cm, inyuswe ngo-0.1V/cm |
| Ubuninzi bangoku | 0.5A |
| Ubuninzi bombane | 350V |
| I-Angle yePulse | ±120° |
| Ixesha leGradiyenti | Umgca |
| Ukutshintsha Ixesha | 50ms ukuya kwi-18h |
| Elona xesha liphezulu lokubaleka | 999h |
| Inani lee-Electrodes | 24, ilawulwa ngokuzimeleyo |
| Uluhlu lobushushu | 0℃ ukuya ku-50℃, imposiso yobhaqo <±0.5℃ |
Inkcazo
I-pulsed field gel electrophoresis (PFGE) yahlula iimolekyuli ze-DNA ngokutshintshanisa intsimi yombane phakathi kwee-electrode pairs ezahlukeneyo zendawo, ezibangela iimolekyuli ze-DNA, ezinokuthi zibe zizigidi zezibini ezisisiseko ezide zilungelelanise kwaye zifuduke nge-agarose gel pores ngezantya ezahlukeneyo. Ifikelela kwisisombululo esiphezulu phakathi kolu luhlu kwaye isetyenziswa ikakhulu kwi-biology yokwenziwa; ukuchongwa kwemigca yebhayoloji kunye ne-microbial; uphando kwi-epidemiology yeemolekyuli; izifundo zamaqhekeza amakhulu eplasmid; ukufumaneka kwendawo yemfuza yezifo; imephu ebonakalayo yezakhi zofuzo, uhlalutyo lwe-RFLP, kunye ne-DNA fingerprinting; uphando lokufa kweeseli olucwangcisiweyo; izifundo ngomonakalo kunye nokulungiswa kwe-DNA; ukuhlukaniswa kunye nohlalutyo lwe-DNA ye-genomic; ukuhlukana kwe-chromosomal DNA; ukwakhiwa, ukuchongwa, kunye nohlalutyo lwamaqhekeza amakhulu amathala eencwadi e-genomic; kunye nophando lwe-transgenic.t ugxininiso olusezantsi njenge-0.5 ng/µL (dsDNA).
Isicelo
Ifanelekile ukufumanisa kunye nokwahlula iamolekyu ze-DNA ukusuka kwi-100bp ukuya kwi-10Mb ngobukhulu, ukufezekisa isisombululo esiphezulu ngaphakathi kolu luhlu.
Uphawu
• ITekhnoloji ePhakamileyo: Idibanisa i-CHEF kunye ne-PACE pulsed-field technologies ukuze kuphunyezwe iziphumo ezizezona zilungileyo ngemizila ethe tye, engagobiyo.
• Ulawulo oluZimeleyo: Iimpawu ze-electrode zeplatinam ezizimeleyo ezingama-24 (i-0.5mm ubukhulu), kunye ne-electrode nganye enokutshintshwa ngokuzimeleyo.
• Umsebenzi wokubala ngokuzenzekela: Udibanisa izinto ezininzi eziguquguqukayo eziphambili ezifana ne-voltage gradient, ubushushu, i-engile yokutshintsha, ixesha lokuqala, ixesha lokuphela, ixesha lokutshintsha langoku, ixesha lokubaleka lilonke, i-voltage, kunye ne-current kubalo oluzenzekelayo, ukunceda abasebenzisi ukuba bafezekise iimeko zovavanyo ezigqwesileyo.
• I-Algorithm ekhethekileyo: Isebenzisa i-algorithm yokulawula i-pulse ekhethekileyo kwimiphumo engcono yokuhlukana, ukwahlula ngokulula phakathi kwe-DNA yomgca kunye nesetyhula, kunye nokwahlula okuphuculweyo kwe-DNA enkulu yesetyhula.
• Ukuzenzekela: Rekhoda ngokuzenzekelayo kwaye iqalise kwakhona i-electrophoresis ukuba inkqubo iphazamisekile ngenxa yokusilela kombane.
Umsebenzisi-oLungisiweyo: Ivumela abasebenzisi ukuseta iimeko zabo.
• Ukuba bhetyebhetye: Isixokelelwano sinokukhetha ukuthambeka kwamandla ombane athile kunye namaxesha okutshintsha kuluhlu oluthile lobungakanani beDNA.
• Isikrini esikhulu: Ixhotyiswe nge-7-intshi ye-LCD yesikrini ukuze isebenze lula, ebonisa ulawulo olulodwa lwesofthiwe yokusetyenziswa okulula kunye nokulula.
• Ukubonwa koBubushushu: Iiprobe ezimbini zobushushu zibhaqa ngokuthe ngqo ubushushu be-buffer kunye nomda wempazamo ongaphantsi kwe ±0.5℃.
• Inkqubo yokujikeleza: Iza nenkqubo yokujikeleza kwe-buffer elawula ngokuchanekileyo kwaye ibeke esweni ubushushu besisombululo se-buffer, iqinisekisa ubushushu obuqhubekayo kunye ne-ionic balance ngexesha le-electrophoresis.
• Ukhuseleko oluPhezulu: Kubandakanya isigqubuthelo sokhuseleko se-acrylic esiselubala esiwacima umbane ngokuzenzekelayo xa siphakanyisiwe, kunye nemisebenzi yokhuseleko olugqithisileyo kunye nokungabikho komthwalo.
• Umgangatho ohlengahlengiswayo: Itanki ye-electrophoresis kunye nejeli ye-caster ibonakalisa iinyawo ezilungelelanisiweyo zokulinganisa.
• I-Mold Design: I-tank ye-electrophoresis yenziwe ngesakhiwo esidibeneyo sokubumba ngaphandle kokudibanisa; i-electrode rack ixhotyiswe nge-electrode ye-platinum eyi-0.5mm, iqinisekisa ukuqina kunye neziphumo zovavanyo ezizinzile.
FAQ
Umbuzo: Yintoni iPulsed Field Gel Electrophoresis?
A: I-Pulsed Field Gel Electrophoresis yindlela esetyenziselwa ukwahlula iimolekyuli ezinkulu zeDNA ngokusekelwe kubukhulu bazo. Kubandakanya ukutshintsha indlela yombane kwi-matrix yejeli ukwenzela ukuba ukwahlulwa kwamaqhekeza e-DNA emakhulu kakhulu ukuba angasonjululwa yi-agarose gel electrophoresis.
Umbuzo: Zeziphi izicelo zePulsed Field Gel Electrophoresis?
A: I-Pulsed Field Gel Electrophoresis isetyenziswa ngokubanzi kwibhayoloji yemolekyuli kunye nemfuzo ye:
Imephu yeemolekyuli ezinkulu zeDNA, ezinjengeechromosomes kunye neeplasmids.
• Ukumisela ubungakanani begenome.
• Ukufunda iiyantlukwano zemfuza kunye nobudlelwane bendaleko.
• I-epidemiology yeemolekyuli, ngakumbi ukulandelela ukuqhambuka kwezifo ezosulelayo.
• Uhlalutyo lomonakalo kunye nokulungiswa kwe-DNA.
• Ukumisela ubukho bemfuza ethile okanye ulandelelwano lweDNA.
Umbuzo: Isebenza njani iPulsed Field Gel Electrophoresis?
A: I-Pulsed Field Gel Electrophoresis isebenza ngokubeka iimolekyuli ze-DNA kwindawo yombane epholileyo etshintshana kwicala. Oku kuvumela iimolekyuli ezinkulu ze-DNA ukuba zilungelelanise kwakhona phakathi kwee-pulses, zivumela ukuhamba kwazo ngejeli matrix. Iimolekyuli ze-DNA ezincinci zihamba ngokukhawuleza ngejeli, ngelixa ezinkulu zihamba kancinci, zivumela ukuhlukana kwazo ngokusekelwe kubukhulu.
Umbuzo: Uthini umgaqo emva kwePulsed Field Gel Electrophoresis?
A: I-Pulsed Field Gel Electrophoresis iyahlula i-molecule ye-DNA ngokusekelwe kubukhulu bayo ngokulawula ubude kunye nolwalathiso lwee-pulses zentsimi zombane. Intsimi etshintshanayo ibangela ukuba iimolekyuli ezinkulu ze-DNA ziqhubeke zilungelelanisa, zikhokelela ekufudukeni kwazo nge-matrix ye-gel kunye nokwahlula ngokobukhulu.
Umbuzo: Ziziphi iingenelo zePulsed Field Gel Electrophoresis?
A: Isisombululo esiphezulu sokwahlula iimolekyuli ezinkulu ze-DNA ukuya kwii-pairs zesiseko sezigidi ezininzi.Ukwazi ukusombulula kunye nokwahlula iziqwenga ze-DNA zobukhulu obufanayo.I-Versatility kwisicelo, ukusuka kwi-microbial typing ukuya kwi-molecular genetics kunye ne-genomics.Indlela esekelwe kwizifundo ze-epidemiological kunye nemephu yezofuzo.
Umbuzo: Ziziphi izixhobo ezifunekayo kwiPulsed Field Gel Electrophoresis?
A: I-Pulsed Field Gel Electrophoresis ifuna i-electrophoresis apparatus ene-electrode ezikhethekileyo zokuvelisa amasimi e-pulsed. Ijeli yeAgarose matrix enogxininiso olufanelekileyo kunye nesithinteli. Unikezelo lwamandla olukwazi ukuvelisa i-high-voltage pulses.Inkqubo yokupholisa ukukhupha ukushisa okwenziwa ngexesha le-electrophoresis, kunye nepompo yokujikeleza.
