I-PCR Thermal Cycler WD-9402M
Iinkcukacha
| Umzekelo | WD-9402M |
| Umthamo | 96×0.2ml |
| Umbhobho | I-96x0.2ml (i-PCR plate ngaphandle / i-semi skirt), i-12x8x0.2ml strips, i-8x12x0.2ml strips, i-0.2ml tubes (ubude 20 ~ 23mm) |
| Block lobushushu Range | 0-105℃ |
| Block lobushushu Ukuchaneka | ±0.2℃ |
| Block Ubushushu Uniformity | ±0.5℃ |
| Iqondo lokutshisa (umndilili) | 4℃ |
| Ireyithi yokupholisa (i-avareji) | 3℃ |
| Ulawulo lobushushu | Block/Tube |
| ITemp yeGradient. Uluhlu | 30-105℃ |
| Iqondo eliphezulu lokufudumeza | 5℃/s |
| Iqondo eliphezulu lokuPholisa 4.5℃ /S | 4.5℃/s |
| I-Gradient Seti i-Span | Max. 42℃ |
| Ukuchaneka kobushushu beGradient | ±0.3℃ |
| Ubushushu bokubonisa ukuchaneka | 0.1℃ |
| Ukufudumeza isivalo Uluhlu lobushushu | 30℃ ~110℃ |
| Ukufudumeza ngokuzenzekelayo isiciko | Cima ngokuzenzekelayo xa isampulu ingaphantsi kwe-30℃ okanye inkqubo ingaphezulu |
| Isibali-xesha siyenyuka / siyehla | -599 ~ 599 S ye-PCR ende |
| Ubushushu Ukunyuka / Ukuhla | -9.9 ~ 9.9℃ ye-Touchdown PCR |
| Isibali-xesha | 1s~59min59sec/ Okungapheliyo |
| Iinkqubo ezigciniweyo | 10000+ |
| Ubuninzi beebhayisikile | 99 |
| Max.Amanyathelo | 30 |
| Misa uMsebenzi | Ewe |
| Touchdown Function | Ewe |
| Umsebenzi wePCR ende | Ewe |
| Ulwimi | IsiNgesi |
| Umsebenzi wokuMisa iNkqubo | Ewe |
| 16℃ Umsebenzi wokuBamba ubushushu | Ayinasiphelo |
| Imo yokusebenza ngexesha lokwenyani | Umbhalo-umfanekiso ubonisiwe |
| Unxibelelwano | I-USB 2.0 |
| Imilinganiselo | 200mm×300mm×170mm (W×D×H) |
| Ubunzima | 4.5kg |
| Ukunikezwa Amandla | 100-240VAC , 50/60Hz, 600W |
Inkcazo
Umjikelo we-thermal usebenza ngokufudumeza ngokuphindaphindiweyo kunye nokupholisa umxube wokuphendula oqukethe i-template ye-DNA okanye i-RNA, i-primers, kunye ne-nucleotides. Ukuhamba ngebhayisikile kweqondo lobushushu kulawulwa ngokuchanekileyo ukufezekisa i-denaturation efunekayo, i-annealing, kunye namanyathelo okwandisa inkqubo ye-PCR.
Ngokuqhelekileyo, umjikelo we-thermal unebhloko equkethe amaqula amaninzi okanye iityhubhu apho umxube wokusabela ubekwe khona, kwaye iqondo lokushisa kwiqula ngalinye lilawulwa ngokuzimeleyo. Ibhloko iyafudunyezwa kwaye ipholile kusetyenziswa iPeltier element okanye enye inkqubo yokufudumeza kunye nokupholisa.
Uninzi lweebhayisikile ezishushu zinonxibelelwano olusebenzisekayo oluvumela umsebenzisi ukuba acwangcise kwaye alungelelanise iiparamitha zokuhamba ngebhayisikile, ezinje ngeqondo lobushushu le-annealing, ixesha elongezelelweyo, kunye nenani lemijikelo. Basenokuba nomboniso wokubeka iliso kwinkqubela phambili yokusabela, kwaye ezinye iimodeli zinokubonelela ngeempawu eziphambili ezifana nolawulo lweqondo lobushushu, uqwalaselo lweebhloko ezininzi, kunye nokubekwa esweni okude kunye nolawulo.
Isicelo
I-Polymerase Chain Reaction (PCR) bubuchule bebhayoloji yemolekyuli esetyenziswa ngokubanzi kwizicelo ezahlukeneyo. Ezinye izicelo eziqhelekileyo zePCR ziquka:
Ukwandiswa kwe-DNA: Eyona njongo iphambili ye-PCR kukukhulisa ulandelelwano oluthile lwe-DNA. Oku kubaluleke kakhulu ekufumaneni izixa ezaneleyo ze-DNA ukuze kuhlalutywe okanye kusetyenziswe imifuniselo.
Uvavanyo lwemfuzo: I-PCR isetyenziswa kakhulu kuvavanyo lwemfuza ukuchonga iimpawu ezithile zofuzo okanye iinguqulelo ezinxulumene nezifo. Ibalulekile kwiinjongo zokuxilonga kunye nokufunda iimeko zofuzo.
I-DNA Cloning: I-PCR isetyenziselwe ukuvelisa izixa ezikhulu zeqhekeza elithile le-DNA, elinokuthi emva koko lidityaniswe libe yi-vector ukuze liqhubeleke okanye lihlalutywe.
Uhlalutyo lwe-DNA yaseForensic: I-PCR ibalulekile kwisayensi yezophando ekwandiseni iisampulu ze-DNA ezifunyenwe kwiindawo zolwaphulo-mthetho. Inceda ekuchongeni abantu ngabanye kunye nokuseka ubudlelwane bemfuza.
Ukufunyanwa kweMicrobial: I-PCR isetyenziselwa ukukhangela iintsholongwane ezincinci kwiisampuli zeklinikhi okanye iisampulu zokusingqongileyo. Ivumela ukuchongwa ngokukhawuleza kwee-arhente ezosulelayo.
I-PCR yobungakanani (i-qPCR okanye i-PCR yexesha langempela): I-qPCR yenza ubungakanani be-DNA ngexesha lenkqubo yokukhulisa. Isetyenziselwa ukulinganisa amanqanaba embonakalo yemfuza, ukufumanisa imithwalo yentsholongwane, kunye nokulinganisa ubungakanani bokulandelelana kwe-DNA ethile.
Izifundo ze-Molecular Evolution: I-PCR isetyenziswa kwizifundo ezivavanya ukwahluka kofuzo phakathi kwabantu, ubudlelwane bendaleko, kunye nohlalutyo lwe-phylogenetic.
Uhlalutyo lwe-DNA yokusiNgqongileyo (i-eDNA): I-PCR iqeshelwe ukukhangela ubukho bezinto eziphilayo ezithile kwiisampulu zokusingqongileyo, igalelo kwizifundo ezahlukeneyo zendalo kunye nendalo.
Ubunjineli bemfuza: I-PCR sisixhobo esibalulekileyo kubunjineli bemfuza ukwazisa ulandelelwano oluthile lwe-DNA kwizinto eziphilayo. Isetyenziswa ekudalweni kwezinto eziphilayo eziguqulwe ngofuzo (GMOs).
ULungiselelo loLungiselelo lweThala leeNcwadi: I-PCR ibandakanyeka kulungiselelo lwamathala eencwadi e-DNA ukwenzela ulandelelwano lweteknoloji yesizukulwana esilandelayo. Inceda ukukhulisa amaqhekeza e-DNA kwizicelo zolandelelwano olusezantsi.
I-Mutagenesis yeSiza: i-PCR isetyenziselwa ukwazisa utshintsho oluthile kwi-DNA ulandelelwano, ivumela abaphandi ukuba bafunde iziphumo zotshintsho oluthile lwemfuza.
DNA Fingerprinting: PCR isetyenziswa ubuchule DNA fingerprinting ukuchongwa ngabanye, uvavanyo paternity, kunye nokuseka ubudlelwane bebhayoloji.
Uphawu
•Imbonakalo entle, ubungakanani obubambeneyo, kunye nesakhiwo esiqinileyo.
•Ixhotyiswe nge-high-performance, ifeni ezolileyo ye-axial-flow ukwenzela inkqubo yokusebenza ezolileyo.
•Ibonisa umsebenzi wokuthambeka obanzi we-30℃, ivumela ukuphuculwa kweemeko zovavanyo ukuhlangabezana neemfuno zovavanyo olungqongqo.
•5-intshi ye-high-definition yombala wescreen esichukumisayo ukwenzela ukuba kube lula kunye nokusebenza ngokulula, okwenza kube lula ukuhlela, ukugcinwa, kunye nokuqhutywa kweenkqubo.
•Inkqubo yokusebenza yodidi lwemizi-mveliso, eququzelela umsebenzi oqhubekayo nongenazimpazamo 7x24.
• Ukudluliselwa kwedatha ngokukhawuleza kwi-USB flash drive ukwenzela ukugcinwa kweprogram elula, ukwandisa umthamo wokugcina idatha.
• I-teknoloji yokupholisa i-semiconductor ephezulu kunye ne-PID ekhethekileyo yokulawula ubushushu beteknoloji iphakamisa ukusebenza ngokubanzi kwiindawo eziphakamileyo ezintsha: ukuchaneka kokulawula ubushushu obuphezulu, ukufudumeza ngokukhawuleza kunye namazinga okupholisa, kunye namaqondo okushisa emodyuli asasazwa ngokufanayo.
FAQ
Umbuzo: Yintoni i-thermal cycler?
A: I-thermal cycler sisixhobo sebhubhoratri esisetyenziselwa ukukhulisa i-DNA okanye i-RNA ulandelelwano nge-polymerase chain reaction (PCR). Isebenza ngebhayisekile ngothotho lotshintsho lobushushu, ivumela ulandelelwano oluthile lwe-DNA ukuba lwandiswe.
Umbuzo: Ngawaphi amacandelo aphambili e-thermal cycler?
A: Amacandelo aphambili e-thermal cycler abandakanya ibhloko yokufudumeza, i-thermoelectric cooler, i-sensors yeqondo lokushisa, i-microprocessor, kunye nepaneli yokulawula.
Umbuzo: Isebenza njani i-thermal cycler?
A: Umjikelo we-thermal usebenza ngokufudumeza kunye nokupholisa iisampulu ze-DNA kuthotho lwemijikelezo yobushushu. Inkqubo yokukhwela ibhayisekile ibandakanya i-denaturation, i-annealing, kunye nezigaba zokwandisa, ngalinye linobushushu obuthile kunye nobude bexesha. Le mijikelo ivumela ulandelelwano oluthile lwe-DNA ukuba lwandiswe nge-polymerase chain reaction (PCR).
Umbuzo: Ziziphi iimpawu ezibalulekileyo ekufuneka ziqwalaselwe xa ukhetha i-thermal cycler?
A: Ezinye izinto ezibalulekileyo ekufuneka ziqwalaselwe xa ukhetha i-thermal cycler ziquka inani lamaqula okanye iityhubhu zokusabela, uluhlu lobushushu kunye nesantya se-ramp, ukuchaneka kunye nokufana kokulawula ubushushu, kunye nojongano lomsebenzisi kunye nobuchule besoftware.
Umbuzo: Uyigcina njani i-thermal cycler?
A: Ukugcina umjikelo we-thermal, kubalulekile ukucoca rhoqo ibhloko yokufudumeza kunye neetyhubhu zokuphendula, khangela ukugqoka kunye nokukrazula kumacandelo, kwaye ulungelelanise i-sensors yeqondo lokushisa ukuqinisekisa ukulawulwa kweqondo lokushisa ngokuchanekileyo kunye nokuhambelana. Kwakhona kubalulekile ukulandela imiyalelo yomenzi wokugcinwa nokulungiswa kwesiqhelo.
Umbuzo: Ngawaphi amanyathelo aqhelekileyo okusombulula ingxaki kumjikelo we-thermal?
A: Amanye amanyathelo aqhelekileyo okusombulula ingxaki kwibhayisikile ye-thermal aquka ukujonga izinto ezikhululekileyo okanye ezonakalisiweyo, ukuqinisekisa ubushushu obufanelekileyo kunye nezicwangciso zexesha, kunye nokuvavanya iityhubhu zokusabela okanye iipleyiti zokungcola okanye umonakalo. Kwakhona kubalulekile ukubhekisela kwimiyalelo yomenzi ngamanyathelo athile okusombulula iingxaki kunye nezisombululo.
