I-PCR Thermal Cycler WD-9402D
Iinkcukacha
| Umzekelo | WD-9402D |
| Umthamo | 96×0.2ml |
| Umbhobho | I-0.2ml ityhubhu, i-8 strips, i-Half skirt96 ipleyiti yamaqula, Akukho siketi 96 ipleyiti yamaqula |
| Umthamo wokusabela | 5-100ul |
| Uluhlu lobushushu | 0-105℃ |
| MAX. Ireyithi yeRamp | 5℃/s |
| Ukufana | ≤±0.2℃ |
| Ukuchaneka | ≤±0.1℃ |
| Isigqibo sokuBonisa | 0.1℃ |
| Ulawulo lobushushu | Block/Tube |
| Ramping Rate Adjustable | 0.01-5℃ |
| ITemp yeGradient. Uluhlu | 30-105℃ |
| Uhlobo lweGradient | I-Gradient eqhelekileyo |
| UkuSasazeka kweGradient | 1-42℃ |
| Ubushushu beSivalo esishushu | 30-115℃ |
| Inani leeNkqubo | 20000 +(USB FLASH) |
| Max. Inombolo yeNyathelo | 40 |
| Max. Inombolo yoMjikelo | 200 |
| Ukwandiswa kwexesha / Ukuncipha | 1 Sec - 600 Sec |
| Ukwandiswa kweqondo lobushushu / ukuhla | 0.1-10.0℃ |
| Misa uMsebenzi | Ewe |
| Ukukhuselwa kwedatha ye-Auto | Ewe |
| Bamba ku-4℃ | Ngonaphakade |
| Touchdown Function | Ewe |
| Umsebenzi wePCR ende | Ewe |
| Ulwimi | IsiNgesi |
| Isoftware yekhompyuter | Ewe |
| Imfonomfono ye-APP | Ewe |
| LCD | 10.1 intshi, 1280×800 iipeliti |
| Unxibelelwano | USB2.0 , WIFI |
| Imilinganiselo | 385mm×270mm×255mm (L×W×H) |
| Ubunzima | 10kg |
| Ukunikezwa Amandla | 100-240VAC , 50/60Hz , 600 W |
Inkcazo
Umjikelo we-thermal usebenza ngokufudumeza ngokuphindaphindiweyo kunye nokupholisa umxube wokuphendula oqukethe i-template ye-DNA okanye i-RNA, i-primers, kunye ne-nucleotides. Ukuhamba ngebhayisikile kweqondo lobushushu kulawulwa ngokuchanekileyo ukufezekisa i-denaturation efunekayo, i-annealing, kunye namanyathelo okwandisa inkqubo ye-PCR.
Ngokuqhelekileyo, umjikelo we-thermal unebhloko equkethe amaqula amaninzi okanye iityhubhu apho umxube wokusabela ubekwe khona, kwaye iqondo lokushisa kwiqula ngalinye lilawulwa ngokuzimeleyo. Ibhloko iyafudunyezwa kwaye ipholile kusetyenziswa iPeltier element okanye enye inkqubo yokufudumeza kunye nokupholisa.
Uninzi lweebhayisikile ezishushu zinonxibelelwano olusebenzisekayo oluvumela umsebenzisi ukuba acwangcise kwaye alungelelanise iiparamitha zokuhamba ngebhayisikile, ezinje ngeqondo lobushushu le-annealing, ixesha elongezelelweyo, kunye nenani lemijikelo. Basenokuba nomboniso wokubeka iliso kwinkqubela phambili yokusabela, kwaye ezinye iimodeli zinokubonelela ngeempawu eziphambili ezifana nolawulo lweqondo lobushushu, uqwalaselo lweebhloko ezininzi, kunye nokubekwa esweni okude kunye nolawulo.
Isicelo
iGenome cloning; Ukulungiswa kwe-PCR ye-asymmetric ye-DNA enentambo enye yokulandelelana kwe-DNA; I-PCR eguqukileyo yokumisela imimandla ye-DNA engaziwayo; reverse transcription PCR (RT-PCR). Ukufumana inqanaba lokubonakaliswa kofuzo kwiiseli, kunye nesixa sentsholongwane ye-RNA kunye nokudibanisa ngokuthe ngqo kwe-cDNA kunye nofuzo oluthile; ukukhulisa ngokukhawuleza iziphelo ze-cDNA; ukufunyanwa kwembonakalo yemfuza; ingasetyenziselwa ukufumanisa izifo zebhaktheriya kunye nentsholongwane; ukuxilongwa kwezifo zofuzo; ukuxilongwa kwamathumba; uphando lwezonyango olufana nobungqina be-forensic ngokomzimba, alunakusetyenziswa kuphando lwezonyango lwezonyango.
Ibonisiwe
• Izinga eliphezulu lokufudumeza kunye nokupholisa, ubuninzi. Isantya sokuhamba 8 ℃/s;
• Ukuqalisa kwakhona ngokuzenzekelayo emva kokusilela kwamandla. Xa amandla ebuyiselwe angaqhubeka nokuqhuba inkqubo engekagqitywa;
• Ukucofa kubekanye ukufukamela okukhawulezileyo kunokuhlangabezana neemfuno zovavanyo ezifana ne-denaturation, i-enzyme cut/enzyme-link kunye ne-ELISA;
• Ubushushu besiciko esishushu kunye nemowudi eshushu yesiciko inokusetwa ukuhlangabezana neemfuno zokulinga ezahlukeneyo;
• Usebenzisa iimodyuli zePeltier zobomi obude obuthile ngebhayisekile;
• Imodyuli ye-aluminiyam e-Anodized kunye nokuqiniswa kobunjineli, egcina ukusebenza komqhubi wokushisa ngokukhawuleza kwaye inokumelana ne-corrosion eyaneleyo;
• Amazinga obushushu akhawulezayo erempu, kunye nesantya esiphezulu serempu eyi-5°C/s, okonga ixesha elixabisekileyo lokulinga;
• I-adaptive pressure bar-style thermal cover, enokuthi ivalwe ngokuqinileyo ngesinyathelo esinye kwaye ikwazi ukuziqhelanisa nokuphakama kweetyhubhu ezahlukeneyo;
• Uyilo lokuhamba komoya ngasemva ukuya ngasemva, ukuvumela ukuba oomatshini babekwe ecaleni;
• Isebenzisa inkqubo yokusebenza ye-Android, ehambelana ne-10.1-intshi ye-capacitive touch screen, ene-graphical menu-style navigation interface, eyenza umsebenzi ube lula kakhulu;
• I-templates yefayile yeprogram eyakhelwe ngaphakathi ye-11, ekwazi ukuhlela ngokukhawuleza iifayile ezifunekayo;
• Ukuboniswa kwexesha langempela lenkqubela phambili yenkqubo kunye nexesha eliseleyo, ukuxhasa inkqubo ephakathi kwesixhobo sePCR;
• Iqhosha elinye elikhawulezayo lokufukamela, lihlangabezana neemfuno zeemvavanyo ezifana ne-denaturation, i-enzyme digestion/ligation, kunye ne-ELISA;
• Iqondo lobushushu elishushu kunye nemowudi yokusebenza yesigqubuthelo esishushu inokusetwa ukuze ihlangabezane neemfuno zovavanyo ezahlukeneyo;
• Ukhuseleko lokucima umbane oluzenzekelayo, ukwenza ngokuzenzekelayo imijikelo engagqitywanga emva kokuba amandla ebuyiselwe, ukuqinisekisa ukusebenza okukhuselekileyo kuyo yonke inkqubo yokukhulisa;
• I-interface ye-USB isekela ukugcinwa kwedatha ye-PCR / ukufunyanwa usebenzisa i-USB drive kwaye ingasebenzisa imouse ye-USB ukulawula isixhobo se-PCR;
• Ixhasa uhlaziyo lwesoftware nge-USB kunye neLAN;
• Imodyuli eyakhelwe-ngaphakathi ye-WIFI, evumela ikhompyutha okanye ifowuni ephathwayo ukuba ilawule ngaxeshanye izixhobo ezininzi zePCR ngoqhagamshelwano lwenethiwekhi;
• Ixhasa isaziso se-imeyile xa inkqubo yovavanyo igqityiwe.
FAQ
Umbuzo: Yintoni i-thermal cycler?
A: I-thermal cycler sisixhobo sebhubhoratri esisetyenziselwa ukukhulisa i-DNA okanye i-RNA ulandelelwano nge-polymerase chain reaction (PCR). Isebenza ngebhayisekile ngothotho lotshintsho lobushushu, ivumela ulandelelwano oluthile lwe-DNA ukuba lwandiswe.
Umbuzo: Ngawaphi amacandelo aphambili e-thermal cycler?
A: Amacandelo aphambili e-thermal cycler abandakanya ibhloko yokufudumeza, i-thermoelectric cooler, i-sensors yeqondo lokushisa, i-microprocessor, kunye nepaneli yokulawula.
Umbuzo: Isebenza njani i-thermal cycler?
A: Umjikelo we-thermal usebenza ngokufudumeza kunye nokupholisa iisampulu ze-DNA kuthotho lwemijikelezo yobushushu. Inkqubo yokukhwela ibhayisekile ibandakanya i-denaturation, i-annealing, kunye nezigaba zokwandisa, ngalinye linobushushu obuthile kunye nobude bexesha. Le mijikelo ivumela ulandelelwano oluthile lwe-DNA ukuba lwandiswe nge-polymerase chain reaction (PCR).
Umbuzo: Ziziphi iimpawu ezibalulekileyo ekufuneka ziqwalaselwe xa ukhetha i-thermal cycler? A: Ezinye izinto ezibalulekileyo ekufuneka ziqwalaselwe xa ukhetha i-thermal cycler ziquka inani lamaqula okanye iityhubhu zokusabela, uluhlu lobushushu kunye nesantya se-ramp, ukuchaneka kunye nokufana kokulawula ubushushu, kunye nojongano lomsebenzisi kunye nobuchule besoftware.
Umbuzo: Uyigcina njani i-thermal cycler?
A: Ukugcina umjikelo we-thermal, kubalulekile ukucoca rhoqo ibhloko yokufudumeza kunye neetyhubhu zokuphendula, khangela ukugqoka kunye nokukrazula kumacandelo, kwaye ulungelelanise i-sensors yeqondo lokushisa ukuqinisekisa ukulawulwa kweqondo lokushisa ngokuchanekileyo kunye nokuhambelana. Kwakhona kubalulekile ukulandela imiyalelo yomenzi wokugcinwa nokulungiswa kwesiqhelo.
Umbuzo: Ngawaphi amanyathelo aqhelekileyo okusombulula ingxaki kumjikelo we-thermal?
A: Amanye amanyathelo aqhelekileyo okusombulula ingxaki kwibhayisikile ye-thermal aquka ukujonga izinto ezikhululekileyo okanye ezonakalisiweyo, ukuqinisekisa ubushushu obufanelekileyo kunye nezicwangciso zexesha, kunye nokuvavanya iityhubhu zokusabela okanye iipleyiti zokungcola okanye umonakalo. Kwakhona kubalulekile ukubhekisela kwimiyalelo yomenzi ngamanyathelo athile okusombulula iingxaki kunye nezisombululo.
