ISikhokelo seNyathelo ngeNyathelo sokuLungiselela i-Agarose Gel ye-Electrophoresis

Ngaba unabo nabuphi na ubunzima ekulungiseleleni ijeli yeagarose?Masilandeleigcisa lethu elebhu ekulungiseleleni ijeli.

Inkqubo yokulungiselela ijeli ye-agarose ibandakanya la manyathelo alandelayo:

Ubunzima beAgarose Powder

Yenza umlinganiselo ofunekayo we-agarose powder ngokogxininiso olufunekayo kuvavanyo lwakho. Uxinzelelo lwe-agarose oluqhelekileyo lusuka kwi-0.5% ukuya kwi-3%. Ugxininiso oluphezulu lusetyenziselwa ukwahlula iimolekyuli ezincinci zeDNA, ngelixa iindawo ezisezantsi zezeamolekyuli ezinkulu.

Ukulungiselela Isisombululo seBhafa

Yongeza i-agarose powder kwi-electrophoresis buffer efanelekileyo, efana ne-1× TAE okanye i-1× TBE. Umthamo wesithinteli kufuneka ungqamane nomthamo wejeli efunekayo kuvavanyo lwakho.

Ukuchithwa kweAgarose

Fudumeza umxube we-agarose kunye ne-buffer de i-agarose inyibilike ngokupheleleyo. Oku kunokwenziwa ngokusebenzisa i-microwave okanye ipleyiti eshushu. Gxuma isisombululo ngokukhawuleza ukukhusela ukuba ungabilisi. Isisombululo se-agarose kufuneka sicace ngaphandle kweengqungquthela ezibonakalayo.

Ukupholisa isisombululo seAgarose

Vumela isisombululo se-agarose eshushu sipholile ukuya kuma-50-60 ° C. Gxuma isisombululo ngexesha lenkqubo yokupholisa ukukhusela ukuqina kwangaphambili.

Ukongeza iNucleic Acid Stain (Ngokhetho)

Ukuba ufuna ukujonga ngeso lengqondo i-DNA okanye i-RNA kwi-gel, unokongeza i-nucleic acid stain, njenge-GelRed okanye i-ethidium bromide, kweli nqanaba. Xa uphethe la mabala, nxiba iiglavu kwaye usebenzise ngononophelo, njengoko zinokuba yingozi.

Ukuphosa iGel

Thela isisombululo se-agarose epholileyo kwi-mold ye-electrophoresis gel mold. Faka ikama ukwenza isampuli yamaqula, uqinisekise ukuba ikama ikhuselekile kwaye isisombululo sisasazwa ngokulinganayo kubumba.

 Ukuqina kweGel

Vumela i-gel ukuba iqine kwiqondo lokushisa, elithatha ngokuqhelekileyo imizuzu engama-20-30 ngokuxhomekeke kugxininiso kunye nobukhulu be-gel.

Rukukhupha iComb

Nje ukuba ijeli yomelele ngokupheleleyo, yisuse ngononophelo ikama ukuze uveze iisampulu yamaqula. Beka i-gel kunye nesikhunta kwigumbi le-electrophoresis kwaye uyigubungele ngexabiso elifanelekileyo le-electrophoresis buffer, uqinisekise ukuba i-gel ifakwe ngokupheleleyo.

Ukulungiselela i-Electrophoresis

Emva kokuba i-gel ilungile, layisha iisampuli zakho emigodini, kwaye uqhubeke novavanyo lwe-electrophoresis.

Ukuba unayo nayiphi na imiba malunga nokulungiselela ijeli, zive ukhululekile ukuqhagamshelana nathi. Sinoochwephesha baselebhu abakhoyo ukuze banxibelelane nawe.

Sichulumancile ukwabelana ngeendaba ezimnandi: itanki yethu edumileyo ye-DYCP-31DN ethe tyaba ye-electrophoresis ikwintengiso ngoku., Qhagamshelana nathi ngolwazi oluthe kratya ngoku!

I-DYCP-31DN itanki ye-electrophoresis ethe tye

I-Beijing Liuyi Biotechnology Co. Ltd (i-Liuyi Biotechnology) ikhethekileyo ekuveliseni izixhobo ze-electrophoresis iminyaka engaphezu kwama-50 kunye neqela lethu lobuchwephesha lobuchwephesha kunye neziko le-R&D. Sinomgca wemveliso othembekileyo nopheleleyo ukusuka kuyilo ukuya ekuhlolweni, kunye nendawo yokugcina impahla, kunye nenkxaso yokuthengisa. Iimveliso zethu eziphambili ziyi-Electrophoresis Cell (itanki / igumbi), i-Electrophoresis Power Supply, i-Blue LED Transilluminator, i-UV Transilluminator, i-Gel Image & Analysis System njl.

Ukuba unayo nayiphi na isicwangciso sokuthenga iimveliso zethu, nceda ungalibazisi ukudibana nathi. Ungasithumela umyalezo nge-imeyile[i-imeyile ekhuselweyo]okanye[i-imeyile ekhuselweyo], okanye nceda usitsalele umnxeba +86 15810650221 okanye wongeze iWhatsapp +86 15810650221, okanye Wechat: 15810650221.

Nceda Skena ikhowudi yeQR ukongeza kuWhatsapp okanye kwiWeChat.