Rizixhobo ofukwenza inwebu:
I-membrane esetyenziselwa i-cellulose acetate electrophoresis ineemfuno ezingqongqo. Okokuqala, imathiriyeli ekrwada yemveliso ye-membrane, i-cellulose acetate, kufuneka ibe nobunyulu obuphezulu. Akufunekanga iqulathe ukungcola okufana ne-hemicellulose, i-lignin, okanye i-ion zentsimbi enzima. Oku kubaluleke kakhulu kukwahlulwa kunye nohlalutyo lweesampulu, njengoko ukufumana iziphumo ezilungileyo zovavanyo kunokuba ngumngeni ngaphandle kwenwebu ecocekileyo. Okwesibini, inwebu kufuneka ibe nengubo efanayo, ubukhulu obufanelekileyo, kunye nokufunxa kakuhle kwamanzi. Ukufezekisa oku, i-membrane kufuneka ifakwe kwicala elinye, ugweme ukubethelwa ngokuphindaphindiweyo ngasemva nangaphandle. Ubukhulu obucetyiswayo be-membrane buphakathi kwe-0.1 ukuya kwi-0.15 mm. Ukuba i-membrane inzima kakhulu, iya kuba nokufunxa kakubi kwamanzi kwaye ibangele ukwahlukana kakubi. Ngakolunye uhlangothi, ukuba i-membrane incinci kakhulu, iyancipha ukuphuka kwaye ayinayo amandla afunekayo omatshini.
Unyango lwangaphambili lwe-membrane ngaphambi kwe-electrophoresis:
Njengoko bekutshiwo ngaphambili, i-cellulose acetate membrane inobudlelwane obuncinci bamanzi xa kuthelekiswa nephepha. Ngoko ke, kubalulekile ukuqinisekisa ukuba inwebu iqulethe isixa esithile sesisombululo se-buffer ngaphambiliukubalekai-electrophoresis. I-membrane kufuneka ifakwe ngaphambili kwisisombululo se-buffer. Indlela echanekileyo yokufunxa inwebu kukuyidada ngobunono kumphezulu wesisombululo se-buffer, ukuvumela inwebu ukuba ifunxe isisombululo se-buffer ukusuka ezantsi kwaye izike kwisisombululo.ukuphepha amaqamza phezu komphezulu inwebu ukuchaphazelaiziphumo zokwahlukana.
Ukongezelela, emva kokuba i-membrane ifakwe emanzini,eyongezelelweyoisisombululo sebuffer kufuneka sisuswe ngobunono kusetyenziswa iphepha lokucoca. Akufunekanga yomiswe ngokupheleleyo okanye ibe manzi kakhulu. Ukuba yome kakhulu, ithintela ukuhlukana kwe-electrophoretic. Ukuba kumanzi kakhulu, kunokuchaphazela ukulayishwa kwesampuli, kubangela ukuba imigca yesampulu isasazeke kwaye iindawo zokuqala zecandelo ngalinye zingalingani, ngaloo ndlela zichaphazela ukusebenza kakuhle kokuhlukana.
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Iinkcukacha ukwazi malunga neBeijing Liuyi Biotechnology's i-cellulose inwebu ye-acetateItanki ye-electrophoresis kunye nesicelo sayo se-electrophoresis, nceda undwendwele apha:
lUlingo lokwahlula iprotein ye-serum nge-Cellulose Acetate Membrane
lI-Cellulose Acetate Membrane Electrophoresis
Ukuba unayo nayiphi na isicwangciso sokuthenga iimveliso zethu, nceda ungalibazisi ukudibana nathi. Ungasithumela umyalezo nge-imeyile[i-imeyile ekhuselweyo]okanye[i-imeyile ekhuselweyo], okanye nceda usitsalele umnxeba +86 15810650221 okanye wongeze iWhatsapp +86 15810650221, okanye Wechat: 15810650221.
Isalathiso:I-Electrophoresis (uhlelo lwesibini) nguMnu Yena kunye noMnu Zhang
Ixesha lokuposa: May-29-2023