Ushwankathelo olufutshaneyeI-Agarose Gel Electrophoresis
I-Agarose gel electrophoresis yindlela esetyenziswa ngokubanzi kwi-biology ye-molecular yokwahlula i-nucleic acids, njenge-DNA kunye ne-RNA, ngokusekelwe kubukhulu bazo. Le ndlela isebenzisa ijeli eyenziwe kwi-agarose, i-polysaccharide yendalo ephuma kwi-seaweed. I-gel isebenza njenge-molecular sieve, ivumela iamolekyu ukuba zifuduke kuyo xa kusetyenziswa umbane. Le nkqubo yokwahlula inceda abaphandi bahlalutye kwaye babonakalise iisampulu ze-nucleic acid ngokujonga iibhendi ezahlukileyo ezenziwe ngokobukhulu bazo. I-Agarose gel electrophoresis sisixhobo esisisiseko semisebenzi efana nohlalutyo lweqhekeza le-DNA, uvavanyo lokucoceka, kunye nophando lwebhayoloji yemolekyuli.
Ngokuqhelekileyo, itanki ye-agarose gel electrophoresis ibizwa ngokuba yi-horizontal electrophoresis tank nayo. Ukusetyenziswa okukhulu kwe-agarose gel electrophoresis kubandakanya:
Uhlalutyo lweqhekeza le-DNA:
Ukwahlula kunye nohlalutyo lwamaqhekeza e-DNA ngokusekelwe kubukhulu, kubalulekile kwimisebenzi efana ne-genotyping kunye ne-DNA fingerprinting.
Uhlalutyo lwe-RNA:
Isisombululo seemolekyuli ze-RNA, ukuncedisa kuphononongo lweepateni zokubonakaliswa kofuzo kunye nokuthembeka kwe-RNA.
Uqinisekiso lweMveliso yePCR:
Ukuqinisekiswa kweemveliso ze-polymerase chain reaction (PCR) ukuqinisekisa ukukhulisa ngempumelelo kunye nokufumana ubungakanani obuchanekileyo beqhekeza.
Uhlalutyo lwePlasmid DNA:
Uvavanyo lwe-plasmid DNA yokulawula umgangatho, ukuqinisekiswa kovavanyo lwe-cloning, kunye nokuchongwa kwe-DNA ephinda iphinde iphinde iphindeke.
UVavanyo lobunyulu:
Ukumiselwa kokucoceka kweesampulu ze-nucleic acid ngokubona izinto ezinokungcolisa okanye ukungcola.
Uphando lwebhayoloji yeMolekyuli:
Isixhobo esisisiseko kwiilebhu zebhayoloji yemolekyuli kwimifuniselo eyahlukeneyo, kubandakanywa amalungiselelo olandelelwano lwe-DNA kunye nokunciphisa i-enzyme yokugaya.
I-Beijing Liuyi Biotechnology Co., Ltd. (eyayisakuba yi-Beijing Liuyi Instrument Factory) yasekwa ngo-1970. Lishishini lobuchwepheshe elisebenza ngokukhethekileyo kwimveliso ye-biochemistry kunye ne-molecular biology laboratory analytical instruments.Liuyi uye wasebenza ngokukhethekileyo ekuveliseni izixhobo ze-electrophoresis iminyaka engaphezu kwama-50 , kwaye inomgca wemveliso othembekileyo kunye opheleleyo ukusuka kuyilo ukuya ekuhlolweni, kunye nendawo yokugcina impahla, kunye nenkxaso yokuthengisa. Iimveliso eziphambili ziyi-Electrophoresis Cell (itanki / igumbi), i-Electrophoresis Power Supply, i-Blue LED Transilluminator, i-UV Transilluminator, i-Gel Image & Analysis System njl.
Sazise ngakumbi ngemodeli yayo enye ye-agarose gel electrophoresis-DYCP-31DN.
Intshayelelo yei-garose gel electrophoresisitanki DYCP-31DN
Ubungakanani (LxWxH) | 310×150×120mm |
Ubungakanani beGel (LxW) | 60×60mm, 60×120mm, 120×60mm, 120×120mm |
Ikama | 2+3 amaqula, 6+3 amaqula, 8+18 amaqula, kunye 11+25 namaqula. |
Ukutyeba kweKamela | 1.0mm, 1.5mm kunye 2.0mm |
Inani leeSampulu | 2-100 |
Buffer Volume | 650ml |
Ubunzima | 1.0kg |
I-DYCP-31DN yeyetanki ye-agarose yejeli ye-electrophoresis ka-Liuyi, enesixhobo sokugalela esifumanekayo sokuphosa iijeli eziyi-1-4. Inokwenza ijeli ezimbini ezincinci njengobungakanani be60x60mm kunye nejeli enye ebanzi 120x60mm okanye ijeli ende 60x120mm ngaxeshanye. Ijeli enkulu enokuthi iphoswe ubukhulu be-120x120cm.
IUbukhulu bekama bunikezelwazi1.0mm, 1.5mm kunye 2.0mm. Ngoyilo lwe-electrode ekhutshwayo, kulula kakhulu ukucoca nokugcina. "Uyilo lweBridge" olujoliswe ekugcineni isisombululo se-buffer ngexesha lenkqubo ye-electrophoresis. Ukusetyenziswa ngokufanelekileyo kwesithinteli kubalulekile ekugcineni iindawo zombane ezizinzileyo kunye nokuqinisekisa ukwahlulwa okuchanekileyo kweeathom.
Amanye amakamva aphambili:
1) Iziciko kunye nemizimba yetanki engundoqo (iitanki zesithinteli) ziyabonakala, zibunjiwe, zintle, ziyahlala, ziyatywinwa kakuhle, azingcoliseki;ayikwazi ukumelana neekhemikhali, ayinyangeki kuxinzelelo.
2) I-Electrodes yenziwe ngeplatinum ecocekileyo (i-quotient yococeko yensimbi ehloniphekileyo ≥99.95%) eneempawu zokumelana nokubola kwe-electroanalysis kunye nokumelana nokushisa okuphezulu, umsebenzi wokuqhuba umbane ulungile kakhulu.
3) Iisampulu yamaqula ahlala kunzima ukuwabona, ngoko ke ibhanti emnyama kwitreyi yejeli yenza kube lula ukujonga kunye nokulayisha iisampuli.
I-4) Amandla ahluleka xa uvula isiciko sokhuseleko lomsebenzisi.
Kukho iimodeli ezininzi zobonelelo lwamandla olubonelelwa nge-DYCP-31DN. Eyona electrophoresis ishushu ukunikezwa Amandla is DYY-6C. Wamkelekile ukuba undwendwele iwebhusayithi yethu kwiimodeli ezininzi zobonelelo lwamandla.
Ukujonga tyenaijeli yeagarose ngeI-DNA okanye i-RNA, uLiuyi ubonelela nge-UV transilluminators kubathengi.WD-9403C, WD-9403B kwayeWD-9403X zonke ziyafumaneka ukuze umthengi azikhethele.
I-Agarose gel electrophoresis iluncedo kwimisebenzi efana nokujonga ubungakanani bamaqhekeza e-DNA, ukuvavanya ukucoceka kwesampulu ye-DNA, okanye ukwahlula amaqhekeza e-DNA ahlukeneyo ukuze kuhlalutywe ngakumbi.
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Ixesha lokuposa: Jan-11-2024