Umgaqo-siseko we-Electrophoresis kunye nezicelo zayo kwiSayensi ye-Biological

I-Electrophoresis yindlela esetyenziselwa ukwahlula i-biomolecules ngokusekelwe kubukhulu bazo kunye nentlawulo usebenzisa intsimi yombane. Isetyenziswa ngokubanzi kwisayensi yebhayoloji ngeenjongo ezahlukeneyo, ukusuka kuhlalutyo lweDNA ukuya ekucoceni iiprotheyini. Apha, sihlolisisa umgaqo we-electrophoresis kunye nezicelo zayo ezahlukeneyo.

Umgaqo we-Electrophoresis

I-Electrophoresis ixhomekeke ekuhambeni kwamasuntswana ahlawuliweyo kwindawo yombane. Ukuseta okusisiseko kubandakanya ukubeka isampuli (equkethe i-biomolecules ehlawuliweyo) kwi-gel okanye kwisisombululo, kunye nokusebenzisa umbane wangoku. I-biomolecules ifuduka phakathi kwinqanaba elihlukeneyo ngokusekelwe kwintlawulo kunye nobukhulu bazo, okukhokelela ekuhlukaneni.

Iintlobo ze-Electrophoresis

1. Gel Electrophoresis

I-Agarose Gel Electrophoresis: Yahlula i-DNA kunye ne-RNA amaqhekeza ngokusekelwe kubukhulu.

I-Polyacrylamide Gel Electrophoresis (PAGE): Isombulula iiprotheyini ezisekelwe kubukhulu kunye nentlawulo.

2. I-Capillary Electrophoresis

Isebenzisa i-capillaries emxinwa yokwahlula, ivumela uhlalutyo olukhawulezayo lwe-DNA, i-RNA, kunye neeprotheni.

Izicelo kwiiSayensi zeBhayoloji

1. Uhlalutyo lweDNA

I-Genotyping: Ichonga ukuhluka kofuzo (umzekelo, ii-SNP) ezinxulumene nezifo.

Ulandelelwano lweDNA: Imisela ulandelelwano lweenucleotides kwimolekyuli yeDNA.

DNA Fragment Analysis: Ubungakanani amaqhekeza DNA izicelo kwibhayoloji molekyuli.

2. Uhlalutyo lwe-RNA

I-RNA Electrophoresis: Ukwahlula iimolekyuli ze-RNA ukuze kuhlalutywe i-gene expression kunye nengqibelelo ye-RNA.

3. Uhlalutyo lweprotheyini

I-SDS-PAGE (i-Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis): Ukwahlula iiprotheni ngokusekelwe kubukhulu.

I-2D Electrophoresis: Idibanisa ukugxila kwe-isoelectric kunye ne-SDS-PAGE ukwahlula iiprotheni ezisekelwe kwindawo ye-isoelectric kunye nobukhulu.

4. Ukusulungekiswa

I-Electrophoresis yokulungiselela: Icoca ii-biomolecules (umzekelo, iiprotheni) ngokusekelwe kwintlawulo kunye nobukhulu.

5. Izicelo zoNyango

IHemoglobin Electrophoresis: Ifumanisa i-hemoglobinopathies (umzekelo, isifo se-sickle cell).

ISerum Protein Electrophoresis: Ichonga izinto ezingaqhelekanga kwiiprotheni zeserum.

6. Izicelo zoPhando

DNA iProfayili: Tshatisa iisampulu zeDNA kuphando lwasenkundleni.

Izinto eziluncedo ze-Electrophoresis

Isisombululo esiphezulu: Ukwahlula i-biomolecules ngokusekelwe kubukhulu kunye nentlawulo ngokuchanekileyo okuphezulu.

I-Versatility: Isebenza kwi-DNA, i-RNA, iiprotheni, kunye nezinye ii-biomolecules ezihlawuliweyo.

Uhlalutyo lobungakanani: Ilinganisa ubuninzi be-biomolecules ngokusekelwe kubukhulu bebhendi.

I-Beijing Liuyi Biotechnology Co. Ltd (i-Liuyi Biotechnology) ikhethekileyo ekuveliseni izixhobo ze-electrophoresis iminyaka engaphezu kwama-50 kunye neqela lethu lobuchwephesha lobuchwephesha kunye neziko le-R&D. Sinomgca wemveliso othembekileyo nopheleleyo ukusuka kuyilo ukuya ekuhlolweni, kunye nendawo yokugcina impahla, kunye nenkxaso yokuthengisa. Iimveliso zethu eziphambili ziyi-Electrophoresis Cell (itanki / igumbi), i-Electrophoresis Power Supply, i-Blue LED Transilluminator, i-UV Transilluminator, i-Gel Image & Analysis System njl.

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