Ukulungiselela i-Agarose Gel ye-Electrophoresis

Ukulungiswa kwe-Agarose Gel ye-Electrophoresis

Qaphela: Soloko unxibe iiglavu ezilahlwayo!

Inyathelo ngenyathelo imiyalelo

Ubungakanani beAgarose Powder:use-weiging paper kunye ne-electronic balance to measure 0.3g ye-agarose powder (esekelwe kwinkqubo ye-30ml).

Ukulungiselela i-TBST Buffer:plungisa i-30ml ye-1x ye-TBST buffer kwi-100ml ye-Erlenmeyer flask.

Ukunyibilikisa umgubo weAgarose:pwethu umgubo weagarose kwi-buffer ye-TBST kwaye uvuthulule kakuhle.Zifakekwi-microwave kunye nobushushu (ngokuqhelekileyo imizuzwana engama-50, egqunywe nge-aluminium foil) ide ichithwe ngokupheleleyo.

Ukupholisa kunye nokongeza iNyukliya:use glavu ukususa umxube kwi-microwave kwaye uyiyeke iphole kancinane emanzini abandayo ide ifudumale (malunga ne-60°C). Yongeza i-2µl ye-nuclease (Eb endaweni yoko) ngelixa ungcangcazela ukuze uxube ngokucokisekileyo.

Ukulungiselela i-Gel Mold:

• Cngqiyame kwaye womise iitreyi yejeli kunye nesixhobo sokuphosa ijelikwitanki ye-electrophoresis.
•  Place ijeliitreyikwitanki yangaphakathi kwaye ufake ikama kwindawo esisigxina.
• Xuba isisombululo sejeli ye-agarose, epholile ukuya kwi-65 ° C, kwaye ugalele ngononophelo kwiitreyi yejelikwiisixhobo sokugalela ijeli, isasaze kancinane de yenze umaleko wejeli olinganayo kwipleyiti yeglasi.
• Vumela ukuba uhlale kwindawo yokushisa kuze kube yilapho i-gel iqina ngokupheleleyo.
• Susa ngobunono ikama ngokuthe nkqo kwaye ususe iteyiphu.
• Beka ijeliitreyikwitanki ye-electrophoresis.

Kubalulekile: Qinisekisa ukuba akukho maqamza omoya kwindawo yamazinyo ekama. Umphezulu wolwelo kufuneka ube mpuluswa ngaphandle kwama-ripples.

Ukubaleka iGel

Ilayisha iGel

Emva kokuba i-gel iqinile, yibeke kwi-tank ye-electrophoresis kwaye uthele i-electrophoresis buffer de i-gel ifakwe emanzini.

Ukulungiselela Iisampulu

• Thatha isiphawuli kunye nokulayisha isithinteli kwisikhenkcisi.
• Yongeza i-6µl yokulayisha isithinteli kwiisampulu kwaye udibanise kakuhle.
• Ukusebenzisa i-micropipette, ngokukhawuleza ulayishe iisampulu kumaqula amakhulu ejeli (qaphela ukuba ungagqobhozi ijeli kwaye ungakhupheli umthamo wonke ukuphepha amaqamza omoya).
• Layisha isiphawuli kuyo nayiphi na imigodi emincinci (khumbula indawo yayo).

Ukuqala i-Electrophoresis

• Gubungela itanki ye-electrophoresis kwaye uqale i-electrophoresis ngokukhawuleza emva kokulayisha i-gel.
• Misela i-voltage kwi-60-100V. Iisampulu ziya kufuduka kwi-electrode engalunganga (emnyama) ukuya kwi-electrode elungileyo (ebomvu).
• Umbane ophezulu unciphisa uluhlu olusebenzayo lokwahlula kwejeli ye-agarose.
• Yeka i-electrophoresis xa idayi ye-bromophenol eluhlaza okwesibhakabhaka ifikelela malunga ne-1cm ukusuka kumda ongezantsi wepleyiti ye-gel.

Ukujonga Iziphumo

Emva kokwahlula iibhendi, yeka i-electrophoresis, khupha i-gel, kwaye uyibone ngokuthe ngqo kwaye uyifote.

Sebenzisa inkqubo yemifanekiso yejeli ukufota kwaye ujonge iibhendi (jonga ukuba kukho nawaphi na amabhanti omakishayo okanye iisampuli).

Emva kokufumana imephu yebhendi yejeli yakho, fumana isiphawuli. Ngokusekwe kwisiphawuli, unokugqiba iibhendi ekujoliswe kuzo!

I-Beijing Liuyi Biotechnology Co. Ltd (i-Liuyi Biotechnology) yenziwe iimveliso ze-electrophoresis iminyaka engaphezu kwama-50. Sibonisa iinkqubo zethu ze-electrophoresis ezinqamlekileyo aphaijeli yeagarosei-electrophoresis.Khethaiinkqubo ze-electrophoresis ezithe tyabaevela eBeijing liuyi yovavanyo lwakho lwejeli ye-electrophoresis ukunceda uphando lwakho kushishino lwebhayoloji.

Sinikezela ngeemodeli ezahlukeneyo zeetanki ze-electrophoresis ezithe tyaba zokuphosa kunye nokusebenza kancinciagaroseijeli ukuya enkuluagaroseiigels

Isikhokelo sokuKhetha seYunithi yeGel eFarisiweyo eMiselweyo eHorizontal

I-Beijing Liuyi Biotechnology Co. Ltd (i-Liuyi Biotechnology) ikhethekileyo ekuveliseni izixhobo ze-electrophoresis iminyaka engaphezu kwama-50 kunye neqela lethu lobuchwephesha lobuchwephesha kunye neziko le-R&D. Sinomgca wemveliso othembekileyo nopheleleyo ukusuka kuyilo ukuya ekuhlolweni, kunye nendawo yokugcina impahla, kunye nenkxaso yokuthengisa. Iimveliso zethu eziphambili ziyi-Electrophoresis Cell (itanki / igumbi), i-Electrophoresis Power Supply, i-Blue LED Transilluminator, i-UV Transilluminator, i-Gel Image & Analysis System njl.

Ukuba unayo nayiphi na isicwangciso sokuthenga iimveliso zethu, nceda ungalibazisi ukudibana nathi. Ungasithumela umyalezo nge-imeyile[i-imeyile ekhuselweyo]okanye[i-imeyile ekhuselweyo], okanye nceda usitsalele umnxeba +86 15810650221 okanye wongeze iWhatsapp +86 15810650221, okanye Wechat: 15810650221.

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