I-Polymerase Chain Reaction (PCR) kunye ne-Gel Electrophoresis: IiTekhnoloji eziBalulekileyo kwiBhayoloji yeMolekyuli

Kwintsimi ehlala iguquguquka ye-biology ye-molecular, i-Polymerase Chain Reaction (PCR) kunye ne-Gel Electrophoresis ziye zavela njengeendlela zembombo eziququzelela ukufundwa kunye nokuguqulwa kwe-DNA. Ezi ndlela zokufundisa azibandakanyi kuphando kuphela kodwa zikwanosetyenziso oluxhaphakileyo kuxilongo, isayensi yophando, kunye ne-biotechnology.

Umfanekiso ovela kwiwebhusayithi ye-biology4alevel

I-PCR yindlela yokuguqula ephuhliswe nguKary Mullis ngo-1983, evumela izazinzulu ukuba zandise icandelo elithile le-DNA ngokukhawuleza. Inkqubo iqala nge-denaturation ye-DNA, apho i-DNA ephindwe kabini ifudumala ukuya kwi-94 ° C, ibangela ukuba ihlukane ibe yimicu emibini enye. Oku kulandelwa yi-annealing, apho iiprimers-ezifutshane ezilandelelanayo ze-nucleotides-zibophelela kulandelelwano oluhambelanayo kwi-DNA enomgca omnye kwiqondo lobushushu elisezantsi (ngokuqhelekileyo malunga ne-55 ° C). Ekugqibeleni, isigaba sokwandisa senzeke kwi-72 ° C, apho i-enzyme ye-DNA polymerase idibanisa i-strand entsha ye-DNA ngokongeza i-nucleotides kwii-primers. Lo mjikelo uphindwa ngamaxesha angama-20-40, okukhokelela kwizigidi zeekopi zokulandelelana kwe-DNA ekujoliswe kuyo.

Beijing LIUYI PCR Machine

Emva kokuba i-DNA ikhulisiwe, i-Gel Electrophoresis iqeshwe ukwahlula kunye nokuhlalutya iimveliso ze-PCR. Obu buchule bubandakanya ukufuduka kwamaqhekeza e-DNA ngokusebenzisa i-agarose gel matrix phantsi kwefuthe lentsimi yombane. Iimolekyuli ze-DNA zihlawuliswa kakubi ngenxa yomqolo we-phosphate, kwaye zifudukela kwi-electrode elungileyo. Ijeli isebenza njengesefu, ivumela amaqhekeza amancinci e-DNA ukuba ahambe ngokukhawuleza kunamakhulu. Ngenxa yoko, amaqhekeza e-DNA ahlukana ngokusekelwe kubukhulu bawo, kunye neebhanti ezicacileyo ezibonakala phantsi kokukhanya kwe-ultraviolet emva kokungcoliswa kwedayi efana ne-ethidium bromide.

EBeijing LIUYIIimveliso zeGel Electrophoresis

Indibaniselwano yePCR kunye neGel Electrophoresis inamandla kwizicelo ezininzi. Kwi-diagnostics yonyango, i-PCR isetyenziselwa ukufumanisa ubukho be-pathogens, ukuguqulwa kofuzo, okanye ulandelelwano oluthile lwe-DNA oluhambelana nesifo. I-Gel Electrophoresis ke ivumela ukubonwa kunye nokuqinisekiswa kwezi ziqwenga ze-DNA ezandisiweyo. Kwinzululwazi yezobungcwalisa, obu buchule bubalulekile kwi-DNA yeminwe, apho inceda ukutshatisa iisampulu ze-DNA ezivela kwiindawo zolwaphulo-mthetho kunye nezo zabarhanelwa.

I-Beijing Liuyi Biotechnology Co. Ltd (i-Liuyi Biotechnology) ikhethekileyo ekuveliseni izixhobo ze-electrophoresis iminyaka engaphezu kwama-50 kunye neqela lethu lobuchwephesha lobuchwephesha kunye neziko le-R&D. Sinomgca wemveliso othembekileyo nopheleleyo ukusuka kuyilo ukuya ekuhlolweni, kunye nendawo yokugcina impahla, kunye nenkxaso yokuthengisa. Iimveliso zethu eziphambili ziyi-Electrophoresis Cell (itanki / igumbi), i-Electrophoresis Power Supply, i-Blue LED Transilluminator, i-UV Transilluminator, i-Gel Image & Analysis System njl.

EBeijing LIUYIIimveliso zeGel Electrophoresis

Ukuba unayo nayiphi na isicwangciso sokuthenga iimveliso zethu, nceda ungalibazisi ukudibana nathi. Ungasithumela umyalezo nge-imeyile[i-imeyile ekhuselweyo]okanye[i-imeyile ekhuselweyo], okanye nceda usitsalele umnxeba +86 15810650221 okanye wongeze iWhatsapp +86 15810650221, okanye Wechat: 15810650221.

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