Polyacrylamide Gel Electrophoresis

I-Polyacrylamide isoloko isetyenziswa kwizicelo zebhayoloji yemolekyuli njengendawo ye-electrophoresis yeeproteni kunye ne-nucleic acids kwindlela eyaziwa ngokuba yiPAGE. Luhlobo lwendlela ye-electrophoresis yendawo ngejeli yokwenziwa ebizwa ngokuba yi-polyacrylamide njengesixhobo esixhasayo. Yakhiwa ngu-S.Raymond kunye no-L.Weintraub ngo-1959, kwaye emva koko yakhuthazwa kwaye yaphuhliswa ngu-L.Ornstein kunye no-BJ Davis. Le ndlela yasetyenziswa ngokubanzi emva kwengcaciso eyongezelelweyo kunye nokuguqulwa ngabo kwithiyori kunye nobuchule bokulinga ngo-1964.

Ngaphambi kokufaka i-polyacrylamide gel electrophoresis, abantu basebenzisa i-electrophoresis yephepha kwi-zone EP. Kodwa iphepha lisebenza njengento ephakathi, inomsebenzi nje wokuchasana nokuhanjiswa, akukho nasinye isiphumo esihle. Ngelixa ijeli ye-polyacrylamide ayinayo kuphela umsebenzi we-anti-convection kodwa inokuthatha inxaxheba ekuqhubekeni kokuhlukana ngokusebenzayo. Ngenxa yokuba ijeli ye-polyacrylamide luhlobo lwesakhiwo somnatha, i-polymerization kunye ne-cross-linking dibaniso ye-acrylamide (Acr) kunye ne-N, N-methylenebis (acrylamide). I-Acrylamide ibizwa ngokuba yi-monomer, ngelixa i-N,N-methylenebis ibizwa ngokuba yi-comonomer okanye i-crosslinker. Ukwenziwa kwejeli yinkqubo yepolymerization yeekhemikhali. Ubungakanani bepore yejeli bunokulawulwa, ngoko ke ijeli inokwenziwa ngeedigri ezahlukeneyo zokudibanisa. Ukuba ubungakanani bepore busondela kumndilili weradiyasi yemolekyuli yesampulu, ukuxhathisa kwemolekyuli ukuya kwipore yejeli kuya kuba nobudlelwane obusondeleyo kunye nobukhulu kunye nokumila kwemolekyuli ngexesha le-electrophoresis. Ke ibonelela ngento eguqukayo yokwahlula ukwahlula ezo mathiriyeli ngeentlawulo ezifanayo.

Kukho iindlela ezimbini eziqhelekileyo ezisetyenziselwa i-polyacrylamide gel electrophoresis, enye yi-disc electrophoresis, kwaye enye yi-slab electrophoresis. I-slab electrophoresis yenzelwe ngokukodwa ukwahlula iprotheyini kunye ne-DNA, kwaye ngokubanzi, kukho iindidi ezimbini ze-slab electrohporesis, itanki ye-electrophoresis ethe tye kunye netanki ye-electrophoresis ethe nkqo. Kwiprotheni, abantu basebenzisa itanki ye-electrophoresis ethe tyaba ye-IFF kunye ne-immunoelectrophoresis, ngaphandle koko, abantu basebenzisa iitanki ze-electrophoresis ezithe nkqo kwiiprotheni.

I-Beijing Liuyi Biotechnology ineentlobo ngeentlobo zeetanki ze-slab electrophoresis zePHEPHA, ngaphandle kohlahlelo kunye nokuchongwa kweesampuli zeprotheni nge-polyacrylamide gel electrophoresis, ingasetyenziselwa ukulinganisa ubunzima beemolekyuli, iisampulu zokucoca kunye nokulungiselela iisampuli.

Thatha imodeliI-DYCZ-23Anjengomzekelo, itanki eqhelekileyo ethe nkqo ye-electrophoresis yeLab. Sebenzisa iipleyiti zeglasi ezimbini ukwenza igumbi lejeli ukwenza ijeli, uze uqhwabe iipleyiti zeglasi ngokuqinileyo ukuthintela ukuvuza kwejeli. Ubukhulu bejeli buxhomekeke kubukhulu be-spacer. Ngokuqhelekileyo, ubukhulu yi-1.5mm yokukhupha ubushushu obungcono, kunyeI-DYCZ-23Aikwabonelela nge-1.0mm ubukhulu bezikhala zokugalela ijeli eyi-1.0mm. Ngaphandle kwetanki ye-electrophoresis, ukuze kuqhutywe i-electrophoresis, ukunikezelwa kwamandla kuyafuneka. I-Beijing Liuyi Biotechnology ibonelela ngeendlela ezahlukeneyounikezelo lwamandla e-electrophoresis. Ukusuka kwi-voltage ephezulu ukuya kwi-voltage esezantsi, ukhetha imodeli ngokwesicelo.

Uphawu lwaseBeijing Liuyi lunembali engaphezulu kweminyaka engama-50 eTshayina kwaye inkampani inokubonelela ngeemveliso ezizinzileyo nezikumgangatho ophezulu kwihlabathi liphela. Ukutyhubela iminyaka yophuhliso, ilufanele ukhetho lwakho!

Ngoku sikhangela amaqabane, zombini itanki ye-OEM electrophoresis kunye nabasasazi bamkelwe.

Ukuze ufumane inkcazelo engakumbi ngathi, nceda uqhagamshelane nathi nge-imeyile[i-imeyile ekhuselweyo]okanye[i-imeyile ekhuselweyo].