Intshayelelo
I-Gel electrophoresis yindlela esisiseko kwibhayoloji yemolekyuli, esetyenziswa ngokubanzi ukwahlula iiproteni, ii-nucleic acids, kunye nezinye iimacromolecules. Ulawulo olululo lwesampulu yesampulu, i-voltage, kunye nexesha le-electrophoresis libalulekile ekufezekiseni iziphumo ezichanekileyo nezinokuphinda zenziwe kwakhona.Ugxa wethu walebhu uyabonelelaiindlela ezilungileyo zokulawula ezi parameters ngexesha le-SDS-PAGE gel electrophoresis.
Beijing Liuyi Biotechnology gel electrophoresis iimveliso
Umthamo weSampuli: Ukuqinisekisa ukuhambelana
Xa wenza i-SDS-PAGE i-electrophoresis, umthamo wesampuli yinto ephambili enokuthi ichaphazele kakhulu isisombululo seziphumo zakho. Kucetyiswa ngokubanzi ukulayisha i-10 µL yeprotheyini iyonke kwiqula ngalinye. Ukuqinisekisa ukuhambelana kunye nokuthintela ukusasazwa kwesampulu phakathi kwamaqula akufutshane, kubalulekile ukulayisha umthamo olinganayo we-1x yokulayisha i-buffer kuwo nawaphi na amaqula angenanto. Oku kuthintela kunceda ukuthintela ukusasazeka kweisampulu kwiindlela ezikufutshane, ezinokuthi zenzeke ukuba iqula lishiywe lingenanto.
Ngaphambi kokulayisha iisampulu zakho, hlala uqala ngokudibanisa i-molecular weight marker kwiqula elinye. Oku kuvumela ukuchongwa ngokulula kobungakanani beeprotheni emva kwe-electrophoresis.
Ulawulo lwe-Voltage: Isantya sokulinganisa kunye neSigqibo
I-voltage esetyenziswe ngexesha le-electrophoresis ichaphazela ngokuthe ngqo zombini isantya apho iisampuli zihamba ngejel kunye nesisombululo sokwahlula. Kwi-SDS-PAGE, kuyacetyiswa ukuba uqale nge-voltage ephantsi ejikeleze i-80V. Lo mbane wokuqala ophantsi uvumela iisampuli ukuba zifuduke kancinci kwaye ngokulinganayo, zigxininise kwibhendi ebukhali njengoko zingena kwijeli eyahlulayo.
Emva kokuba iisampuli zingene ngokupheleleyo kwijeli yokwahlula, i-voltage inokunyuswa ukuya kwi-120V. Lo mbane uphezulu ukhawulezisa ukufuduka, uqinisekisa ukuba iiproteni zahlulwe ngokufanelekileyo ngokobunzima bemolekyuli. Kubalulekile ukujonga inkqubela phambili yedayi ye-bromophenol eluhlaza okwesibhakabhaka, ebonisa ukugqitywa kwe-electrophoresis. Kwii-gels kunye ne-concentration ye-10-12%, imizuzu ye-80-90 ngokuqhelekileyo yanele; nangona kunjalo, kwi-15% yejeli, unokufuna ukwandisa ixesha lokugijima kancinci.
Ulawulo Lwexesha: Ukwazi Ixesha Lokuyeka
Ixesha lelinye ixesha elibalulekileyo kwi-gel electrophoresis. Ukusebenzisa ijeli ixesha elide kakhulu okanye elifutshane kakhulu kunokukhokelela ekuhlukaneni okungafanelekanga. Ukufuduka kwedayi ye-bromophenol eluhlaza okwesibhakabhaka luphawu oluncedo: xa lufikelela ngaphantsi kwe-gel, ngokuqhelekileyo lixesha lokuyeka ukugijima. Kwiigels eziqhelekileyo, ezifana ne-10-12%, ubude be-electrophoresis malunga nemizuzu engama-80-90 ngokuqhelekileyo ngokwaneleyo. Kwipesenti ephezulu yeegels, njenge-15%, ixesha lokugijima kufuneka landiswe ukuze kuqinisekiswe ukuhlukana okupheleleyo kweeprotheni.
Ulawulo lweSithinteli: UkuSebenzisa kwakhona kunye nokuLungisa izithinteli
Isithinteli se-Electrophoresis singaphinda sisetyenziswe amaxesha angama-1-2, ngokuxhomekeke kwiimeko ezithile zelebhu yakho. Nangona kunjalo, ngeziphumo ezizezona zilungileyo, kuyacetyiswa ukuba ulungiselele i-10x buffer entsha kwaye uyingxengxe phambi kokusetyenziswa. Oku kuqinisekisa ukuba i-buffer igcina ukusebenza kwayo, okukhokelela kwiziphumo ezithembekileyo ze-electrophoresis.
Beijing Liuyi Biotechnology gel electrophoresis iimveliso
Ngokulawula ngononophelo umthamo wesampulu, i-voltage, kunye nexesha le-electrophoresis, unokuphucula kakhulu ukuchaneka kunye nokuphindaphinda iziphumo zakho ze-gel electrophoresis. Ukuphumeza ezi zenzo zingcono kumsebenzi wakho webhubhoratri kuya kukunceda ufezekise ngokucacileyo kunye namaqela ahlukeneyo, okukhokelela kwidatha engcono yohlalutyo olusezantsi.
Ukuba uneendlela ezilungileyo zokwandisa ilinge le-gel electrophoresis, wamkelekile ukuxoxa nathi!
I-Beijing Liuyi Biotechnology Co. Ltd (i-Liuyi Biotechnology) ikhethekileyo ekuveliseni izixhobo ze-electrophoresis iminyaka engaphezu kwama-50 kunye neqela lethu lobuchwephesha lobuchwephesha kunye neziko le-R&D. Sinomgca wemveliso othembekileyo nopheleleyo ukusuka kuyilo ukuya ekuhlolweni, kunye nendawo yokugcina impahla, kunye nenkxaso yokuthengisa. Iimveliso zethu eziphambili ziyi-Electrophoresis Cell (itanki / igumbi), i-Electrophoresis Power Supply, i-Blue LED Transilluminator, i-UV Transilluminator, i-Gel Image & Analysis System njl.
Ukuba unayo nayiphi na isicwangciso sokuthenga iimveliso zethu, nceda ungalibazisi ukudibana nathi. Ungasithumela umyalezo nge-imeyile[i-imeyile ekhuselweyo]okanye[i-imeyile ekhuselweyo], okanye nceda usitsalele umnxeba +86 15810650221 okanye wongeze iWhatsapp +86 15810650221, okanye Wechat: 15810650221.
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Ixesha lokuposa: Aug-20-2024