Xa kusenziwa uhlalutyo lokuthelekisa iziphumo ze-electrophoresis, izinto ezininzi zinokukhokelela kumahluko kwiidatha:
Ukulungiselela Isampulu:Ukwahluka kwisampulu yoxinaniso, ukucoceka, kunye nokuthotywa kunokuchaphazela iziphumo ze-electrophoresis. Ukungcola okanye i-DNA / i-RNA eyonakeleyo kwisampulu inokubangela ukuba i-smearing okanye i-non-specific bands.
Ukugxilwa kweGel kunye noHlobo:Ukugxininiswa kunye nohlobo lwejeli (umzekelo, i-agarose okanye i-polyacrylamide) ichaphazela isisombululo sokuhlukana kweemolekyuli. Iigel zoxinzelelo eziphakamileyo zingcono ukwahlula iimolekyuli ezincinci, ngelixa ii-gel zoxinzelelo ezisezantsi zifanelekile kwiimolekyuli ezinkulu.
Iimeko ze-Electrophoresis:Amandla ombane (i-voltage), ixesha le-electrophoresis, kunye nodidi kunye ne-pH ye-buffer esebenzayo inokuchaphazela zonke iziphumo. Ukuphakama kakhulu kwamandla ombane kunokubangela ukuba ibhendi ibe yintsila okanye kuncitshiswe isisombululo, kwaye ixesha elongeziweyo le-electrophoresis lingakhokelela ekusasazweni kwebhendi.
Umgangatho kunye noLungiselelo lwe-Buffer:Izithinteli ezingafanelekanga okanye eziphelelwe lixesha zingakhokelela kutshintsho kwi-pH kunye namandla e-ionic, ezichaphazela ukuhamba kweemolekyuli kunye nesisombululo.
Isampulu yokuLayisha iSixa kunye nobuChwephesha:Ukulayisha ngaphezulu okanye ukulayishwa kweesampulu kunokuchaphazela ukucaca kwebhendi kunye nokuqina. Ukulayisha okungalinganiyo kunokukhokelela kwisampulu yosasazo okanye iindlela ezigoso.
Isixhobo se-Electrophoresis kunye neeMeko zokusiNgqongileyo: Izixhobo ezahlukeneyo ze-electrophoresis (ezifana neetanki ze-gel kunye nezixhobo zombane) kunye neemeko zokusingqongileyo (ezifana nokushisa kunye nomswakama) zinokuchaphazela ukuzinza kunye nokuphindaphinda kweziphumo ze-electrophoresis.
Iindlela zokungcolisa kunye nokubona:Ukukhethwa kwebala (umzekelo, i-ethidium bromide, i-SYBR eluhlaza) kunye nexesha lokungcolisa kunokuchaphazela ukucaca kunye nokubonwa kweebhendi.
Umgangatho weGel ye-Electrophoresis:Amabhamuza kwiijeli zasekhaya, umgangatho wejeli ongalinganiyo, okanye ijeli ezonakeleyo zinokubangela ukuba iibhendi zigobe okanye zifuduke ngokungaqhelekanga.
Ubume kunye nobukhulu be-DNA/RNA:Ingaba i-DNA okanye i-RNA kwisampulu i-linear, i-circular, okanye i-supercoiled, okanye ubukhulu beqhekeza, iya kuchaphazela isantya sabo sokufuduka kwi-gel.
IMbali yokubamba iSampuli:Izinto ezifana nenani lemijikelo yokunyibilika komkhenkce, ubushushu bokugcina, kunye nobude bexesha kunokuchaphazela imfezeko yesampulu, ngaloo ndlela kube nefuthe kwiziphumo ze-electrophoresis.
Ingcali ye-Liuyi Biotechnology yenza umfuniselo we-electrophoresis elebhu
Wamkelekileukunxibelelana nathi ukuxoxa ngezinto ezinokubangela ukungafani kwedatha ye-electrophoresis. Ngokuqonda le miba, sinokunciphisa ukuguquguquka kwedatha, siphucula ukuphindaphinda kovavanyo kunye nokuchaneka kweziphumo..
I-Beijing Liuyi Biotechnology Co. Ltd (i-Liuyi Biotechnology) ikhethekileyo ekuveliseni izixhobo ze-electrophoresis iminyaka engaphezu kwama-50 kunye neqela lethu lobuchwephesha lobuchwephesha kunye neziko le-R&D. Sinomgca wemveliso othembekileyo nopheleleyo ukusuka kuyilo ukuya ekuhlolweni, kunye nendawo yokugcina impahla, kunye nenkxaso yokuthengisa. Iimveliso zethu eziphambili ziyi-Electrophoresis Cell (itanki / igumbi), i-Electrophoresis Power Supply, i-Blue LED Transilluminator, i-UV Transilluminator, i-Gel Image & Analysis System njl.
Ukuba unayo nayiphi na isicwangciso sokuthenga iimveliso zethu, nceda ungalibazisi ukudibana nathi. Ungasithumela umyalezo nge-imeyile[i-imeyile ekhuselweyo]okanye[i-imeyile ekhuselweyo], okanye nceda usitsalele umnxeba +86 15810650221 okanye wongeze iWhatsapp +86 15810650221, okanye Wechat: 15810650221.
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Ixesha lokuposa: Sep-12-2024