Ijeli ayicwangciswanga ngokufanelekileyo
Umba: Ijeli ineepateni okanye ayilingani, ngakumbi kwiigels eziphezulu zokuxilisa ngexesha lobushushu obubandayo basebusika, apho umzantsi wejeli eyahlulayo ibonakala i-wavy.
Isisombululo: Ukwandisa inani le-polymerizing agents (i-TEMED kunye ne-ammonium persulfate) ukukhawulezisa inkqubo yokumisela. Qinisekisa ukuba iipleyiti zeglasi zicocekile ukuthintela iintsalela kwiijeli zangaphambili.
Ijeli yiFragile
Umba: Iigels ezisezantsi (ezisetyenziselwa iiprotheyini ezinkulu ze-molecular weight) ziyaphuka ngexesha lokuphatha.
Isisombululo: Phatha ijeli ngobunono kuyo yonke inkqubo. Kwiimeko ezibandayo, ukwandisa kancinci inani le-polymerizing agents.
Ijeli ayibeki
Isisombululo: Kwiqondo lokushisa eliphantsi, ukwandisa inani le-polymerizing agents. Qinisekisa ukuba i-ammonium sulfate ilungiswe ngokutsha. Ukuba ingxaki iyaqhubeka, phinda lungisa isisombululo se-buffer.
Imijelo emininzi kunye ne-Smears kwi-Gel Emva kwe-Electrophoresis
Isisombululo: Qinisekisa ukuba isisombululo sejeli sicocekile kwaye sixutywe kakuhle. Lawula ukusetyenziswa kwakhona kwe-electrophoresis buffer okanye uphephe ukuphinda uyisebenzise ngokupheleleyo. Ukuba ukonga kwisithinteli, sebenzisa isithinteli esitsha kwigumbi elingaphakathi kunye nesithinteli esiphinda sisetyenziswe kwigumbi elingaphandle.
Ixesha lokuSeta iGel elingalunganga
Umba: Iijeli zasekhaya zisetwa ngaphakathi kwemizuzu engama-30 ukuya kwiyure, ngelixa iikhithi zorhwebo zisetwa ngaphakathi kwemizuzu eyi-10-20. Ukuba useto lucotha kakhulu, idosi ye-arhente ye-polymerizing inokwanela. Ukuba ukusetwa kukhawuleza kakhulu, i-dosage ingaba phezulu kakhulu, yenza i-gel ibe nzima kwaye ikwazi ukuqhekeka, kunye nokwandisa umngcipheko wokushisa ngexesha le-electrophoresis.
Isisombululo: Lungisa inani leejenti ze-polymerizing ngokusekelwe kwiimeko zangempela. Ixesha lokumisa ijeli lichaphazeleka kakhulu ngubushushu.
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Kukho iindidi ngeendidi zeetanki ze-electrophoresis ezithe nkqo zeprotheyini ye-electrophoresis yokuhlalutya kunye nokuchongwa kweesampulu zeprotheni nge-polyacrylamide gel electrophoresis, kunye nokulinganisa ubunzima beemolekyuli, iisampuli zokucoca kunye nokulungiselela iisampuli. Ezi mveliso zonke zamkelwe kwimarike yasekhaya naphesheya.
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Ixesha lokuposa: Jul-29-2024