Apha siza kuchaza indlela yokwenza i-agarose gel electrophoresis ngumphandi wethu kwilebhu ye-Liuyi Biotech.
Ngaphambi kovavanyo, kufuneka sijonge izixhobo, ii-reagents, kunye nezinye izixhobo zovavanyo kunye nezixhobo esizidingayo.
Ukulungiswa kwezixhobo zokulinga kunye nemathiriyeli
Izixhobo ze-agarose gel electrophoresis
Iseli ye-electrophoresis ethe tyaba (itanki / igumbi), umbane ophakathi kunye nosezantsi wombane we-electrophoresis, umfanekiso wejeli kunye nenkqubo yokuhlalutya.
I-Beijing liuyi Biotechnology Co., Ltd (i-Liuyi Biotech) inikezela ngeemodeli ezahlukeneyo zeseli ye-electrophoresis ethe tyaba (itanki / igumbi) kunye nonikezelo lwamandla kunye nenkqubo yamaxwebhu ejeli. Imodeli yeemveliso ze-DYCP-31 zeze-electrophoresis, kunye neemveliso ze-DYY ze-electrophoresis power supply. Iimveliso ze-WD-9413 ziyinkqubo yoxwebhu lwejeli. Ukusuka kubungakanani bejeli encinci 60 × 60mm ukuya kwijeli enkulu 250 × 250mm, sinokuhlangabezana neemfuno zakho ezahlukeneyo zobungakanani bejeli. ImodeliI-DYCP-32Cinokwenza ijeli ifikelele kubungakanani be250×250mm. IDYY-6Clubonelelo lwethu lwamandla. Ixhasa imveliso ye-400V, 400mA, 240W, eyimveliso yethu eqhelekileyo esetyenziswa ngabathengi bethu. WD-9413BI-Gel Image & Analysis System isetyenziselwa ukuhlalutya kunye nophando lwejel, iifilimu, kunye nama-blots emva kovavanyo lwe-electrophoresis. Sisixhobo esisisiseko esinomthombo wokukhanya we-ultraviolet wokujonga kunye nokufota iijeli ezidyojwe ngedayi ekhanyayo efana ne-ethidium bromide, kunye nomthombo wokukhanya omhlophe wokujonga kunye nokufota iijeli ezidyojwe ngedayi efana ne-coomassie ebhlowu eqaqambileyo.
Ii-reagents ze-agarose gel electrophoresis
1.Iphakathi: ijeli yeagarose
2.I-buffer: TAE (i-Tris-acetate, i-EDTA, i-glacial acetic acid) kunye ne-TBE (i-Tris-borate, i-EDTA, i-boric acid).
3.Isithinteli sokulayisha: I-6 × i-DNA yokuLayisha i-Buffer (I-Special for DNA sample: EDAT, glycerine, Xylene cyanol kunye ne-bromophenol blue)
Idayi
Idayi yefluorescent efana ne-EB, Gelred, goldview, GenGreen, GenView, SYBRGreen
IIzinto ezisetyenziswayo
Iingcebiso zepipette yokuvala inzala (10μL), iingcebiso zepipette (200μL), iingcebiso zepipette (1000μL), 200μL\500μL\1.5ml ityhubhu yecentrifuge.
DNA umakisha
Ilungiselelwe ngokobunzima bemolekyuli ekufuneka ivavanywe.
Ijeli iyabaleka kwaye ijonge
Okokuqala, kufuneka silungiselele ijeli ye-agarose. Ukugxininiswa kwe-agarose kwijel kuya kuxhomekeka kubukhulu beeqhekeza ze-DNA ukuba zihlulwe, kunye neegels ezininzi ziphakathi kwe-0.5% -2%. Ukuthatha wethuI-DYCP-31DNumzekelo, Ungaphosa ijeli encinci, ijeli ebanzi, ijeli ende kunye nejeli yesikwere ngokweemfuno zovavanyo. Khetha itreyi yejeli oyifunayo, kwaye ufake ikama, emva koko Galela ijeli yeagarose eshushu kwisixhobo sokugalela ijeli.
Emva koko, emva kokuba ijeli yomelele, thatha ikama uye kude ngocoselelo nangokuthambileyo, uze ubeke itreyi yejeli kwitanki yesithinteli. Galela isisombululo sebuffer kwitanki yesithinteli kwaye wenze yonke ijeli intywiliselwe kwisithinteli. Layisha iisampuli emigodini kunye nepipette eqhelekileyo. Qhagamshela iI-DYCP-31DNkunye nonikezelo lwamandla e-electrophoresis ngokuchanekileyo, kwaye usethe iiparamitha zokuqhuba ijeli.
Yiba nombono wamaqhekeza e-DNA yakho kwi-UV transilluminator.
AEmva kokusebenzisa ijeli, ungasebenzisa umfanekiso wethu wejeli kunye nemodeli yenkqubo yohlalutyoWD-9413Bukujonga, ukuhlalutya kunye nokuthatha imifanekiso yejeli. I-Liuyi Biotech ikwabonelela nge-UV transilluminator (UV Analyzer) ukujonga ijeli. Sinebhokisi emnyama uhlobo lwe-UV trimodeli ye-ansilluminator (UV Analyzer).WD-9403A, 9403C, WD-9403F, imodeli ephathekayo ye-UV transilluminator (UV Analyzer).WD-9403Bkunye nokubamba ngesandla i-UV transilluminator (I-UV Analyzer)WD-9403Ekuba ukhetha.
Umfanekiso we-gel post electrophoresis
I-brand ye-Liuyi ineminyaka engaphezu kwama-50 yembali e-China kwaye inkampani inokubonelela ngeemveliso ezizinzileyo nezikumgangatho ophezulu kwihlabathi jikelele. Ukutyhubela iminyaka yophuhliso, ilufanele ukhetho lwakho!
Ukuze ufumane inkcazelo engakumbi ngathi, nceda uqhagamshelane nathi nge-imeyile[i-imeyile ekhuselweyo], [i-imeyile ekhuselweyo].
Ixesha lokuposa: Apr-29-2022