Yintoni iDNAukulandelelana?
Yinkqubo yokumiselaulandelelwano oluchanekileyo okanye ulandelelwano lweziseko (A, C, G no T) kwimolekyuli yeDNA.Kutheni kufuneka sazi indlela iDNA elandelelana ngayo kwimizila yemfuza ethile? Apha siyazi ezinye izicelo. Okokuqala, kunokusinceda sibone utshintsho kwimizila yemfuza. Ukuba siyalwazi ulandelelwano lwe-DNA, kuya kusinceda ukuba sahlule into ephilayo kwenye into ephilayo.iihaplotypeskunye dukukhupha iipolymorphisms.
Kubaluleke ngokwenene kwaye kuluncedo ukuphanda ubuchule bokulandelelana kwe-DNA. EBeijing Liuyi Biotechnology Co., Ltd, sibonelela ngezixhobo ze-DYCZ-20 zechungechunge lwe-electrophoresis ukunceda abaphandi ukuba baphonononge ukulandelelana kwe-DNA, uhlalutyo lweminwe ye-DNA, ukuboniswa ngokwahlukileyo njl.
Kutshanje, igcisa kwilebhu yethu ukubonisa indlela yokuphosa ijeli ngesixhobo sethu se-DYCZ-20F electrophoresis. Igcisa uMnu.Wu wabelane ngeendlela zokutofa ijeli kwigumbi leglasi.
Isixhobo se-DYCZ-20F se-electrophoresis sinokuphosa ubukhulu bejeli obuyi-340x450mm. Okokuqala, kufuneka silungiselele wonke umsebenzi esiwudingayo ukuphosa ijeli, njengesisombululo sejel, gisitya sokulala,gel ibhotile, amazinyo kakrebe, i-spacer(0.4mm), iikliphu.
Ngaphambi kokuba silahle i-gel, kufuneka sicoce kwaye silungise iplate yeglasi kuqala. Apha, umvavanyi ucebise ukusebenzisa amanzi ukucoca umphezulu weepleyiti zeglasi. Emva koko gquba i-Bind -silane kumphezulu wepleyiti yeglasi ukunceda ijeli ibambelele kwipleyiti yeglasi, kwaye unxibe iRepel-silane kwiplate yeglasi.hayi ipleyiti yeglasi ukuxobula ijeli ngokulula.
Umlingi ubonise iindlela ezimbini zokutofa isisombululo sejeli:
Nciphisa kancinci igumbi leglasi yejeli, kwaye ujove isisombululo sejeli ngokulinganayo emva naphambili. (Amaqamza omoya kufuneka aphetshwe ukuvelisa ngexesha lale nkqubo. )
Ukuba umntu ozamayo ukhetha le ndlela, kufuneka aziqhelanise rhoqo ukuze aphumelele ngale ndlela.
Kwabaqalayo, sincoma le ndlela yokuphosa i-gel. Qhwaba ngamacala amathathu ngeziqeshana, kwaye ujikelise igumbi leglasi yejeli kwi-engile ye-45 degree, emva koko utofe isisombululo sejeli kwicala lakho lasekunene de isisombululo sejeli sizaliswe kwigumbi lejeli lonke.
Ndinqwenela ukuba lo mboniso wejeli unokunceda umthengi wethu ukuba alungiselele uvavanyo lwabo.
I-Beijing Liuyi DYCZ-20 uchungechunge lwe-DNA yohlalutyo lokulandelelana kwesixhobo se-electrophoresis sisetyenziselwa ukukhangela ukuguqulwa kofuzo. Ukuguqulwa kofuzo yenye yezona zinto zibangela izifo ezininzi zofuzo. Nge-electrophoresis ehlalutya i-DNA kwisampulu, ukuguqulwa kofuzo kulandelelwano lwemfuza kunokubonwa, ngaloo ndlela kuncede oogqirha babone ukuba ngaba isigulane sithwala isifo esibangela isifo. Olu ngcelele lusenokusetyenziswa kwi-genotyping. I-Genotyping ibhekiselele kuhlalutyo lweloci ethile kwigenome yomntu ukuze kubonwe uhlobo lwegenotype yabo. Oku kubalulekile kubaphandi kuba ii-genotypes ezahlukeneyo zinokunxulunyaniswa nokuba sesichengeni kwizifo ezithile okanye ukuphendula ngamachiza. Ngokusebenzisa i-Liuyi DNA yohlalutyo lokulandelelana kwesixhobo se-electrophoresis, i-genotyping inokwenziwa ngokukhawuleza nangokuchanekileyo, inika isiseko esibalulekileyo kwiyeza lomntu. Ukongeza, olu luhlu lwezixhobo lungasetyenziselwa ukulandelelana kweDNA. Ukulandelelana kwe-DNA yindlela ebalulekileyo yokuba abaphandi baqonde isakhiwo kunye nomsebenzi we-genome. Ngokubeka iisampulu ze-DNA kuhlalutyo lwe-electrophoretic, ukulandelelana kweziseko kulandelelwano lwe-DNA kunokumiselwa, ukunceda abaphandi batolike ulwazi kwi-genome. Wamkelekile ukuba uqhagamshelane nathi ukufumana ulwazi oluthe vetshe iimveliso.
Ngoku sikhangela amaqabane, zombini itanki ye-OEM electrophoresis kunye nabasasazi bamkelwe.
Ukuba unayo nayiphi na isicwangciso sokuthenga iimveliso zethu, nceda ungalibazisi ukudibana nathi. Ungasithumela umyalezo nge-imeyile[i-imeyile ekhuselweyo]okanye[i-imeyile ekhuselweyo], okanye nceda usitsalele umnxeba +86 15810650221 okanye wongeze iWhatsapp +86 15810650221, okanye Wechat: 15810650221
Ixesha lokuposa: Oct-12-2023