I-DNA Gel Electrophoresis: Ukuhlalutya i-Genetic Fragments

Ijeli ye-DNA ye-electrophoresis yindlela eqhelekileyo yebhayoloji ye-molekyuli esetyenziselwa ukwahlula kunye nokuhlalutya amaqhekeza e-DNA ngokusekelwe kubukhulu bawo. Le nkqubo ibandakanya ukulayisha amaqhekeza e-DNA anobungakanani obahlukeneyo kwijeli eyenziwe ngeagarose, icarbohydrate efumaneka kwialgae ebomvu.

Ukulungiselela kunye nokuphosa ijeli ye-agarose

1. Nyibilikisa i-agarose kwisixa esifanelekileyo se-electrophoresis buffer. Ukuxinwa kwejeli kugqitywa ngumlinganiselo we-mass-to-volume, njenge-1g ye-agarose kwi-100.ml ye-buffer ye-1% yejeli.
2. Fudumeza umxube kwi-microwave, ujikelezisa isitya ukuze uqinisekise ukuchithwa ngokupheleleyo kwe-agarose.
3. Yongeza i-ethidium bromide kwisisombululo se-gel kwi-concentration yokugqibela ye-0.5mg/ ml. I-Ethidium bromide idibanisa phakathi kweeperi ezikufutshane ze-DNA kwaye ikhupha i-orenji fluorescence phantsi kokukhanya kwe-UV. Qaphela ukuba i-ethidium bromide yi-carcinogen, ngoko ke ukuyiphatha kufuna amanyathelo afanelekileyo okhuseleko afana nokunxiba iiglavu.
4. Ukupholisa isisombululo sejeli kwindawo yokuhlambela amanzi ukukhusela i-tray yejeli ukuba ingaguquki ngenxa yokushisa okuphezulu.
5. Beka ikama kwisisombululo sejeli ukwenza isampuli yamaqula. Khetha iikama ezilungele isixa sesampulu yeDNA oza kuyilayisha. Galela isisombululo se-agarose gel kwi-itreyi yejelikwaye uyivumele ukuba iqine kwiqondo lokushisa.
6. Emva kokuba ijeli iqinile, susa ikama. Ukuba awuzukusebenzisa ijeli ngoko nangoko, yisongele ngeplastiki kwaye uyigcine ku-4℃ de ifuneke.

Ukulungiselela kunye nokuqhuba ijeli

Ngaphambi kokuba uqalise i-electrophoresis, xuba isampuli ye-DNA kunye nokulayisha isithinteli. I-buffer yokulayisha idla ngokuphindwe kathandathu ngakumbi kwaye inceda isampuli ye-sink ukuya ezantsi kwamaqula kwaye ilandele intshukumo ngexesha le-electrophoresis.

Misela unikezelo lwamandla kumbane ochaziweyo.

Yongeza i-electrophoresis buffer eyaneleyo kwitanki yejeli ukugquma umphezulu wejeli.Qinisekisauqhagamshelwano oluchanekileyo lwe-electrode.

Layisha isampuli ye-DNA kunye neziphawuli zobunzima bemolekyuli kumaqula ejeli.

Vula unikezelo lwamandla ukuqalisa i-electrophoresis.

Ukujonga amaqhekeza e-DNA ahlukeneyo

Emva kwe-electrophoresis, cima umbane kwaye ususe i-gel.

Sebenzisa umthombo wokukhanya we-UV ukukhanyisa ijeli; Amaqhekeza e-DNA aya kuvela njengeebhanti eziorenji ze-fluorescent.

Bhala umfanekiso wejeli ukuhlalutya amaqhekeza e-DNA ahlukeneyo.

Emva kovavanyo, qinisekisa ukulahlwa ngokufanelekileyo kwejeli kunye ne-electrophoresis buffer, ngokulandela izikhokelo zaselabhoratri. Ngalo lonke ixesha unxibe iiglavu xa uphethe iijeli kunye nezithinteli ezineethidium bromide ukukhusela ngokuchasene nokuvezwa kule nto iyingozi.

Ijeli ye-DNA ye-electrophoresis isetyenziswa kakhulu kwibhayoloji yemolekyuli kunye nophando lwemfuzo ukuqikelela ubungakanani bemolekyuli yeDNA, ukwahlula amaqhekeza eDNA, ukufumanisa ukuguqulwa kofuzo, kunye nokuprintwa kweminwe yeDNA, phakathi kwezinye izicelo. Bubuchule obulula nobusebenzayo bokulinga obunceda ekuqondeni ukwakheka kunye neempawu zeesampulu zeDNA.

I-Beijing Liuyi Biotechnology Co.Ltd, umenzi oqeqeshiweyo ogxile kwiimveliso ze-electrophoresis kwiminyaka engama-50, unikezela ngoluhlu lwamatanki e-electrophoresis (amagumbi / iiseli) ze-gel electrophoresis, ezifana neitanki ze-electrophoresis ezinqamlekileyo (amagumbi / iiseli)DNAi-gel electrophoresis, kunye neetanki ze-electrophoresis ezithe nkqo (amagumbi / iiseli) ze-protein gel electrophoresis. Okwangoku, ikwabonelela ngeentlobo ezahlukeneyo zobonelelo lwamandla e-electrophoresis, isihlalutyi sebhokisi emnyama yekhabhathi kunye ne-UV transilluminator. Unokukhetha amatanki e-electrophoresis eBeijing Liuyi (amagumbi/iiseli) ukulinganisa ubunzima bemolekyuli ye-RNA kunye nokwahlula i-RNA yobukhulu obahlukeneyo kwilebhu yakho.

Apha sizacebisauhlobo olulodwa lwetanki ye-electrophoresis ethe tye (igumbi / iseli) imodeliI-DYCP-31DN ukwenza kunye nokuqhuba ijeli.

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Ngoku sikhangela amaqabane, zombini itanki ye-OEM electrophoresis kunye nabasasazi bamkelwe.

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