I-Comet Assay: I-Technique e-Sensitive yokuFumana umonakalo we-DNA kunye nokulungiswa

I-Comet Assay (i-Gel ye-Gel ye-Electrophoresis enye, i-SCGE) yindlela enovakalelo kunye neyokukhawuleza isetyenziswe ngokuyinhloko ukufumanisa umonakalo we-DNA kunye nokulungiswa kweeseli nganye. Igama elithi "Comet Assay" livela kwi-comet-like shape ebonakala kwiziphumo: i-nucleus yeseli yenza "intloko," ngelixa i-DNA fragments eyonakalisiweyo ifuduka, idala "umsila" ofana ne-comet.

Umgaqo

Umgaqo we-Comet Assay usekelwe ekufudukeni kwamaqhekeza e-DNA kwindawo yombane. I-DNA ecocekileyo ihlala ngaphakathi kwinucleus yeseli, ngelixa yonakeleyo okanye i-DNA eqhekezayo ifudukela kwi-anode, yenze “umsila” we-comet. Ubude kunye nobukhulu bomsila buhambelana ngokuthe ngqo nobungakanani bomonakalo we-DNA.

Inkqubo

1. Ukulungiselela iSeli: Iiseli eziza kuvavanywa zixutywe ne-low-melting-point agarose kwaye zisasazwa kwi-microscope izilayidi ukuze zenze umaleko ofanayo.
2. Iseli Lysis: Izilayidi zintywiliselwa kwisisombululo se-lysis ukususa i-membrane yeseli kunye ne-nuclear membrane, eveza i-DNA.
3. Electrophoresis: Izilayidi zifakwe kwigumbi le-electrophoresis phantsi kweemeko ze-alkaline okanye ezingathathi hlangothi. Iziqwenga zeDNA ezonakeleyo zifudukela kwi-electrode elungileyo phantsi kwefuthe lendawo yombane.
4. Ukungcolisa: Emva kwe-electrophoresis, iislayidi zenziwe ngedayi ye-fluorescent (umzekelo, i-ethidium bromide) ukujonga i-DNA.
5. Uhlalutyo lweMicroscopic: Ukusebenzisa i-microscope ye-fluorescence okanye isofthiwe ekhethekileyo, iimilo ze-comet zihlaziywa, kwaye iiparitha ezifana nobude bomsila kunye nobukhulu ziyalinganiswa.

Umfanekiso ovela kwi-biorender

Uhlalutyo lwedatha

Iziphumo ezivela kwiComet Assay zivavanywa ngokusekwe kwiiparamitha ezininzi eziphambili:

• Ubude bomsila: Imele umgama we-DNA efudukayo, ebonisa ubungakanani bomonakalo weDNA.
• Umxholo weDNA yomsila: Ipesenti yeDNA efudukela emsileni, edla ngokusetyenziswa njengomlinganiselo wobungakanani bomonakalo weDNA.
• Umzuzu womsila we-Olive (OTM): Idibanisa ubude bomsila kunye nomsila we-DNA ukubonelela ngomlinganiselo obanzi womonakalo we-DNA.

Usetyenziso

1. Izifundo zeGenotoxicity: I-Comet Assay isetyenziswa ngokubanzi ukuvavanya imiphumo yeekhemikhali, iziyobisi, kunye nemitha ye-radiation kwi-DNA yeseli, okwenza kube sisixhobo esibalulekileyo sovavanyo lwe-genotoxicity.
2. I-Toxicology yendalo: Inceda ekuhlalutyeni ifuthe longcoliseko lokusingqongileyo kwi-DNA yezinto eziphilayo, inika ulwazi kukhuseleko lwe-ecosystem.
3. Uphando lwezonyango kunye noNyango: I-Comet Assay isetyenziselwa ukufunda iindlela zokulungisa i-DNA, umhlaza, kunye nezinye izifo ezinxulumene ne-DNA. Ikwavavanya impembelelo yonyango lomhlaza njengeradiotherapy kunye nechemotherapy kwiDNA.
4. IiSayensi zoKutya nezoLimo: Isetyenziselwa ukuvavanya ukhuseleko lwezitshabalalisi, izongezo zokutya, kunye nezinye izinto, kunye nokufunda iziphumo zazo eziyityhefu kwiimodeli zezilwanyana.

Iingenelo

• Uvakalelo oluPhezulu: Iyakwazi ukufumanisa amanqanaba aphantsi omonakalo weDNA.
• Ukusebenza okulula: Ubuchwephesha buchanekile, bubenza bufanelekele ukukhangela okuphezulu.
• Usetyenziso Olubanzi: Ingasetyenziswa kwiintlobo ezahlukeneyo zeeseli, kubandakanya izilwanyana kunye neeseli zezityalo.
• Imingeni yobungakanani: Ngelixa ukubonelela ngedatha esemgangathweni kumonakalo weDNA, uhlalutyo lobungakanani luxhomekeke kakhulu kwisoftware kunye nobuchule bokuhlalutya umfanekiso.
• Iimeko zoMfuniselo: Iziphumo zinokuphenjelelwa yimiba efana nexesha le-electrophoresis kunye ne-pH, efuna ukulawulwa ngenyameko kweemeko zovavanyo.

Ukulinganiselwa

I-Comet Assay sisixhobo esixabisekileyo kuphando lwe-biomedical, isayensi yendalo, kunye nophuhliso lweziyobisi ngenxa yokuguquguquka kwayo kunye novakalelo oluphezulu ekuboneni umonakalo kunye nokulungiswa kwe-DNA. Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology)inikeza igumbi le-electrophoresis elithe tye kwi-comet assay. Wamkelekile ukuba uqhagamshelane nathi ukuze sixoxe malungaComet Assayiprotocol.

I-Beijing Liuyi Biotechnology Co. Ltd (i-Liuyi Biotechnology) ikhethekileyo ekuveliseni izixhobo ze-electrophoresis iminyaka engaphezu kwama-50 kunye neqela lethu lobuchwephesha lobuchwephesha kunye neziko le-R&D. Sinomgca wemveliso othembekileyo nopheleleyo ukusuka kuyilo ukuya ekuhlolweni, kunye nendawo yokugcina impahla, kunye nenkxaso yokuthengisa. Iimveliso zethu eziphambili ziyi-Electrophoresis Cell (itanki / igumbi), i-Electrophoresis Power Supply, i-Blue LED Transilluminator, i-UV Transilluminator, i-Gel Image & Analysis System njl.

Ukuba unayo nayiphi na isicwangciso sokuthenga iimveliso zethu, nceda ungalibazisi ukudibana nathi. Ungasithumela umyalezo nge-imeyile[i-imeyile ekhuselweyo]okanye[i-imeyile ekhuselweyo], okanye nceda usitsalele umnxeba +86 15810650221 okanye wongeze iWhatsapp +86 15810650221, okanye Wechat: 15810650221.

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