I-Cellulose Acetate Membrane Electrophoresis

Yintoni iCellulose Acetate Membrane Electrophoresis?

I-Cellulose Acetate Membrane Electrophoresis lolunye uhlobo lweendlela ze-electrophoresis esebenzisa i-cellulose acetate membrane njengemithombo yeendaba exhasayo kwimifuniselo.

I-Cellulose Acetate luhlobo lwe-acetate ye-cellulose ene-acetylated esuka kwi-cellulose's hydroxyl. Xa i-soluble kwisisombululo sezinto eziphilayo ezifana ne-acetone, inokugqunywa kwifilimu edibeneyo kunye nelungileyo kunye ne-micro-pores. Ubungqingqwa befilimu bujikeleze i-0.1-0.15mm elungele ukulinga.

Iinzuzo ze-cellulose acetate membrane

Xa kuthelekiswa nephepha le-fliter, linamancedo alandelayo:

1.Isiphumo esihle sokwahlukana. Kwiisampulu zeprotheyini, i-cellulose acetate membrane ifunxa ezimbalwa kuzo, emva kwedayi, inokutshintsha ngokupheleleyo umbala ongemva. Iibhendi zedayi zicace kakhulu, ngoko ke ikhuthaza ukuchaneka kokuzimisela kobungakanani.
2.Fast kwaye wonge ixesha. I-cellulose acetate inwebu ingaphantsi kwe-hydyophilic kunephepha le-fliter, ngoko ke ukusebenza kwe-electro-osmosis kungaphantsi kwephepha le-fliter, kwaye umbane wangoku uqhutywa ikakhulu ziisampuli ezinokufutshane ixesha lokwahlukana kunye nexesha elingaphantsi kwe-electrophoresis. Ngokuqhelekileyo, ixesha le-cellulose acetate membrane electrophoresis li malunga ne-45-60minutes. Yonke inkqubo ye-electrophoresis kubandakanywa ukudaya kunye nokuchithwa kwemibala kufuna kuphela imizuzu engama-90.
3.Uvakalelo oluphezulu kunye neesampuli ezincinci eziza kusetyenziswa. Ifuna kuphela i-2 μL serum kwi-serum protein electrophoresis.
4.Usetyenziso olubanzi. Ezinye iiprotheyini azilula ukuhlukana kwiphepha le-electrophoresis, njenge-Alpha-fetoprotein, i-bacteriolytic enzyme, i-insulin, kunye ne-histone njl. Kodwa kungcono ngakumbi ukusebenzisa i-cellulose i-acetate membrane electrophoresis ukubahlula.
5.Easy ukugcina kunye quantification. Emva kokudaya iisampulu ze-cellulose acetate inwebu ye-electrophoresis, ntywilisela iisampulu kwisisombululo esixutyiweyo se-glacial acetic acid kunye ne-ethanol ukwenza ipleyiti ecacileyo eyomileyo egcinwe ixesha elide.

Ngenxa yokuba i-cellulose acetate membrane electrophoresis kulula ukuyisebenzisa, ngoku isetyenziswa ngokubanzi kuhlalutyo kunye nokuvavanya iprotheni yeplasma, i-lipoprotein, i-glycoprotein, i-Alpha-fetoprotein, i-enzyme, i-polypeptide, i-nucleic acid kunye nezinye i-biomacromolecule.

Ukulungiselela iCellulose Acetate Membrane Electrophoresis

Izixhobo ezisetyenzisiweyo ziquka umbane ophantsi kwamandla ombane, itanki ye-electrophoresis ethe tyaba. Imidiya exhasayo yi-cellulose acetate membrane.

Ii-reagents:

I-1.pH8.6 isisombululo se-barbitol buffer: i-Diethyl barbituric acid, i-Diethyl sodium pentobarbital;
2.Ibala:Ponceau S, Trichloroacetic;
I-3.TBS / T okanye i-PBS / T: I-95% ye-ethanol, i-glacial acetic acid;
I-4.Isisombululo sokucoca: i-ethanol e-anhydrous, i-glacial acetic acid.

I-Beijing Liuyi Biotechnology Co., i-ltd inenkqubo evuthiweyo ye-cellulose acetate membrane electrophoresis. Imodeli ye-DYCP-38C ligumbi elithambileyo lephepha kunye ne-electrophoresis yesilayidi, kunye ne-cellulose acetate membrane electrophoresis. Umzimba oyintloko uyabunjwa, akukho nto yokuvuza. Imodeli yonikezelo lwamandla i-DYY-2C, i-DYY-6C, i-DYY-6D, i-DYY-8C, i-DYY-10C kunye ne-DYY-12 inokubonelela ngamandla kwi-DYCP-38C. Ngokubanzi, umthengi uthanda ukukhetha imodeli ye-DYY-6C njengobonelelo lwamandla lwe-DYCP-38C.

Njengemveliso efunekayo ye-DYCP-38C ye-electrophoresis tank, i-Liuyi ikwabonelela nge-cellulose acetate membrane. Kukho ubungakanani obuthathu obuqhelekileyo be-membrane, 7 * 9cm, 2 * 8cm kunye ne-12 * 8cm, kwaye sinokubonelela ngobungakanani obulungiselelweyo be-cellulose acetate membrane njengeemfuno zakho ngokunjalo.

Amanqaku malunga nomfuniselo:

1.Layisha isampuli kwicala elibi: i-1-2UL ifanelekile
2.I-electrophoresis eqhubekayo yangoku: amandla angoku 0.4～0.6m A/cm
I-3.pH8.6 isisombululo se-barbitol buffer: Amandla e-Ionic 0.06
I-4.Ixesha lokudaya: imizuzu emi-5 ngokwaneleyo
5.Ukugcinwa: faka imephu ye-elecrtophoresis eyomileyo kwisisombululo sokucoca kwi-10-15 imizuzu, uze uyikhuphe kwaye unamathele kwiglasi ecocekileyo, emva kokuba yomile, iya kuba yimephu yefilimu ebonakalayo.

I-brand ye-Liuyi ineminyaka engaphezu kwama-50 yembali e-China kwaye inkampani inokubonelela ngeemveliso ezizinzileyo kunye neziphezulu emhlabeni jikelele.

Ukuze ufumane inkcazelo engakumbi ngathi, nceda uqhagamshelane nathi nge-imeyile[i-imeyile ekhuselweyo], [i-imeyile ekhuselweyo].