Ukulungiselela iSampuli kunyeIyalayisha
Ngenxa yokusetyenziswa kwenkqubo ye-buffer eqhubekayo ngaphandle kokupakisha ijeli kwiimeko ezininzi, iisampuli kufuneka zibe noxinzelelo olufanelekileyo kunye nomthamo omncinci. Sebenzisa aipayipiukongeza ngokucothayo isampuli, kunye ne-5-10 μg kwiqula ngalinye, ukunqanda ukuhla okubalulekileyo kwisisombululo. Niniukulayisha isampulu, unikezelo lwamandla kufuneka lucinywe. Isampuli kufuneka iqulethe idayi yesalathisi (i-0.025% ye-bromophenol eluhlaza okwesibhakabhaka okanye i-orange) kunye ne-sucrose (10-15%) okanye i-glycerol (5-10%) yokwandisa ubuninzi bayo, igxininise isampuli, kwaye inqande ukusabalalisa. Nangona kunjalo, ngamanye amaxesha i-sucrose okanye i-glycerol inokubangela ukuba i-U-shaped bands kwiziphumo ze-electrophoresis, ezinokuphetshwa ngokusebenzisa i-2.5% ye-Ficoll (i-polyvinylpyrrolidone).
Electrophoresis
I-voltage ye-electrophoresis yi-5-15 V / cm, ngokubanzi malunga ne-10 V / cm. Ukwahlula iimolekyuli ezinkulu, i-voltage kufuneka ibe ngaphantsi, ngokuqhelekileyo ingadluli kwi-5 V / cm.
Ukungcolisa
Idayi yeFluorescent ethidium bromide (EB) iqhele ukusetyenziselwa ukungcolisa ukujonga iibhanti zeDNA kwijeli yeagarose. I-EB inokufaka phakathi kweeperi ezisezantsi zee-molecule ze-DNA, ezibangela ukuba i-EB ibophe kunye ne-DNA. Ukuxutywa kwe-260 nm ukukhanya kwe-ultraviolet nge-DNA idluliselwa kwi-EB, kwaye i-EB eboshiweyo ikhupha i-fluorescence kwi-590 nm kummandla obomvu-orenji we-spectrum yokukhanya okubonakalayo. Ukungcolisa i-gel kwisisombululo se-1 mmol / L MgSO4 kwiyure ye-1 kunokunciphisa i-fluorescence yangasemva ebangelwa yi-EB engavumelekanga, iququzelele ukufunyanwa kweemali ezincinci ze-DNA.
Idayi ye-EB ineenzuzo ezininzi: kulula ukuyisebenzisa, ayiphuli i-nucleic acids, inovakalelo oluphezulu, kwaye inokungcolisa zombini i-DNA kunye ne-RNA. I-EB inokongezwa kwisampulu kwaye ilandelelwe ngokusebenzisa i-UV yokufunxa nangaliphi na ixesha. Emva kokungcola, i-EB inokususwa ngokutsalwa nge-n-butanol.
Nangona kunjalo, idayi ye-EB yi-mutagen enamandla, kwaye izilumkiso, ezifana nokugqoka iiglavu ze-polyethylene, kufuneka zithathwe ngexesha lokuphatha. I-Acridine orange nayo idayi esetyenziswa ngokuqhelekileyo kuba iyakwazi ukwahlula phakathi kwe-nucleic acids enye kunye ne-double-stranded nucleic acids (DNA, RNA). Ibonisa i-fluorescence eluhlaza (i-530 nm) ye-nucleic acids ephindwe kabini kunye ne-fluorescence ebomvu (i-640 nm) ye-nucleic acids enemisonto enye. Ukongezelela, ezinye iidayi ezifana ne-methylene blue, i-methylene eluhlaza, kunye ne-quinoline B zingasetyenziselwa ukungcolisa.
I-Beijing Liuyi Biotechnology Co. Ltd (i-Liuyi Biotechnology) ikhethekileyo ekuveliseni izixhobo ze-electrophoresis iminyaka engaphezu kwama-50 kunye neqela lethu lobuchwephesha lobuchwephesha kunye neziko le-R&D. Sinomgca wemveliso othembekileyo nopheleleyo ukusuka kuyilo ukuya ekuhlolweni, kunye nendawo yokugcina impahla, kunye nenkxaso yokuthengisa. Iimveliso zethu eziphambili ziyi-Electrophoresis Cell (itanki / igumbi), i-Electrophoresis Power Supply, i-Blue LED Transilluminator, i-UV Transilluminator, i-Gel Image & Analysis System njl.
Ngoku sikhangela amaqabane, zombini itanki ye-OEM electrophoresis kunye nabasasazi bamkelwe.
Ukuba unayo nayiphi na isicwangciso sokuthenga iimveliso zethu, nceda ungalibazisi ukudibana nathi. Ungasithumela umyalezo nge-imeyile[i-imeyile ekhuselweyo]okanye[i-imeyile ekhuselweyo], okanye nceda usitsalele umnxeba +86 15810650221 okanye wongeze iWhatsapp +86 15810650221, okanye Wechat: 15810650221.
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Ixesha lokuposa: Dec-20-2023