Gene Electroporator GP-3000
Iinkcukacha
| Umzekelo | GP-3000 |
| IFomu yePulse | Ukubola kwe-Exponential kunye ne-Square Wave |
| Ukukhutshwa kwamandla aphezulu | 401-3000V |
| Ukukhutshwa kwamandla aphantsi | 50-400V |
| I-capacitor yombane ophezulu | 10-60μF kumanyathelo e-1μF (10μF, 25μF, 35μF, 50μF, 60μF iyacetyiswa) |
| I-capacitor yombane ophantsi | 25-1575μF kumanyathelo ayi-1μF (amanyathelo angama-25μF acetyiswayo) |
| Isixhasi esihambelanayo | 100Ω-1650Ω kwi 1Ω amanyathelo (50Ω iyacetyiswa) |
| Ukunikezwa Amandla | 100-240VAC50/60HZ |
| Inkqubo yokwenza | Ulawulo lweMicrocomputer |
| Ixesha alitshintshi | kunye nexesha le-RC elingaguqukiyo, lihlengahlengiswa |
| Ukusinda okushiyekile | 4.5kg |
| Iipakethi Imilinganiselo | 58x36x25cm |
Inkcazo
I-electroporation yeseli yindlela ebalulekileyo yokwazisa ii-macromolecules zangaphandle ezifana ne-DNA, i-RNA, i-siRNA, iiprotheni, kunye neemolekyuli ezincinci kumbindi wenwebu zeseli.
Ngaphantsi kwempembelelo yentsimi yombane eyomeleleyo okwethutyana, i-membrane yeseli kwisisombululo ifumana ukunyanzeliswa okuthile. Izinto ezihlawuliswayo zangaphandle zingena kwi-membrane yeseli ngendlela efana ne-electrophoresis. Ngenxa yokuxhathisa okuphezulu kwe-phospholipid bilayer ye-membrane yeseli, i-bipolar voltages eveliswa yintsimi yangoku yombane yangaphandle ithwalwa yi-membrane yeseli, kwaye i-voltage esasazwa kwi-cytoplasm inokungahoywa, phantse akukho okwangoku kwi-cytoplasm, ngaloo ndlela kwakhona ukugqiba ubuthi obuncinci kuluhlu oluqhelekileyo lwenkqubo ye-electrophoresis.
Isicelo
Ingasetyenziselwa i-electroporation ukudlulisa i-DNA kwiiseli ezifanelekileyo, iiseli zezityalo kunye nezilwanyana, kunye neeseli zegwele. Okufana ne-electroporation yeebhaktheriya, igwele, kunye nezinye ii-microorganisms, ukudluliselwa kweeseli ze-mammalian, kunye nokudluliselwa kwezicubu zezityalo kunye neeprotoplasts, i-cell hybridization kunye ne-gene fusion introduction, ukuqaliswa kweejene zokumakisha zokubhala kunye neenjongo zokubonisa, ukuqaliswa kweziyobisi, iiprotheni, izilwa-buhlungu, kunye nezinye iimolekyuli zokufunda ukwakheka nokusebenza kweeseli.
Uphawu
• Ukusebenza okuphezulu: ixesha elifutshane lokuguqulwa, izinga eliphezulu lokuguqulwa, ukuphindaphinda okuphezulu;
• Ukugcinwa okunengqondo: kunokugcina iiparamitha zokulinga, ezilungele abasebenzisi ukuba basebenze;
• Ulawulo oluchanekileyo: i-microprocessor-controlled pulse discharge;Ø
• Ukubonakala okumangalisayo: uyilo oludibeneyo lomatshini wonke, umboniso ocacileyo, ukusebenza okulula.
FAQ
Umbuzo: Yintoni iGene Electroporator?
A: I-Gene Electroporator sisixhobo esisetyenziselwa ukwazisa izinto zemfuza zangaphandle, ezifana ne-DNA, i-RNA, kunye neeprotheni, kwiiseli ngenkqubo ye-electroporation.
Umbuzo: Ziziphi iintlobo zeeseli ezinokujoliswa kwi-Gene Electroporator?
A: I-Gene Electroporator ingasetyenziselwa ukwazisa izinto zofuzo kwiintlobo ezahlukeneyo zeeseli ezibandakanya ibhaktheriya, imvubelo, iiseli zezityalo, iiseli ze-mammalian, kunye nezinye i-microorganisms.
Umbuzo: Zeziphi izicelo eziphambili zeGene Electroporator?
A:
• I-electroporation yebhaktheriya, yeast, kunye nezinye ii-microorganisms: Ukuguqulwa kofuzo kunye nezifundo ze-gene function.
• Ukutshintshwa kweeseli zezilwanyana ezincancisayo, izicubu zezityalo, kunye neeprotoplasts: Kuhlalutyo lwembonakalo yemfuza, i-genomics esebenzayo, kunye nobunjineli bemfuza.
• Ukuxutywa kweeseli kunye nentshayelelo yokudityaniswa kofuzo: Ukwenza iiseli ezixubeneyo kunye nokwazisa imfuzo edibeneyo.
• Ukwaziswa kohlobo lofuzo olumakishayo: Ukufaka iilebhile kunye nokulandelela imbonakalo yejini kwiiseli.
• Ukwaziswa kwamachiza, iiprotheyini, kunye nezilwa-buhlungu: Ukuphanda ubume beseli kunye nokusebenza, ukuhanjiswa kweziyobisi, kunye nezifundo zokusebenzisana kweeprotheyini.
Umbuzo: Isebenza njani iGene Electroporator?
A: I-Gene Electroporator isebenzisa i-pulse emfutshane, ephezulu-voltage yombane ukudala ii-pores zexeshana kwi-membrane yeseli, ivumela iimolekyuli ezingaphandle ukuba zingene kwiseli. I-membrane yeseli iphinda ibuyele emva kwe-pulse yombane, ibambe iimolekyuli ezifakwe ngaphakathi kwiseli.
Umbuzo: Ziziphi iingenelo zokusebenzisa i-Gene Electroporator?
A: Ukuphindaphinda okuphezulu kunye nokusebenza kakuhle, ukulula ukusebenza: Inkqubo elula kwaye ekhawulezayo, ulawulo lobuninzi, akukho genotoxicity: Umonakalo omncinci onokuthi ube khona kwizinto zofuzo zeseli.
Umbuzo: Ngaba i-Gene Electroporator ingasetyenziselwa zonke iintlobo zovavanyo?
A: Ngelixa i-Gene Electroporator inezinto ezininzi, ukusebenza kwayo kunokuhluka ngokuxhomekeka kuhlobo lweseli kunye nezinto zofuzo eziziswayo. Kubalulekile ukunyusa iimeko zovavanyo oluthile ngalunye.
Umbuzo: Yiyiphi inkathalo ekhethekileyo efunekayo emva kokwaziswa?
A: Ukunyamekela emva kokungeniswa kunokubandakanya ukufukamela iiseli kwindawo yokubuyisela ukuze zibancede balungise kwaye baphinde baqalise imisebenzi eqhelekileyo. Iinkcukacha zinokwahluka ngokuxhomekeke kuhlobo lweseli kunye novavanyo.
Umbuzo: Ngaba kukho nayiphi na inkxalabo yokhuseleko ngokusebenzisa i-Gene Electroporator?
A: Iindlela ezisemgangathweni zokhuseleko lwaselabhoratri kufuneka zilandelwe. I-Gene Electroporator isebenzisa amandla ombane aphezulu, ngoko ke ukuphathwa ngokufanelekileyo kunye neenkqubo zokhuseleko kufuneka zithotyelwe ukuze kuthintelwe iingozi zombane.
