DYCZ-24DN Icwecwe leglasi eliNotshileyo (1.5mm)

I-DYCZ – I-24DN iseli ye-electrophoresis encinci ye-mini ye-electrophoresis encinci yeyohlalutyo olukhawulezayo lweprotheyini kunye neesampulu ze-nucleic acid kwi-polyacrylamide encinci kunye neegels ze-agarose. Indlela yejeli ethe nkqo intsonkothe ​​kancinci kunomlingani wayo othe tye. Inkqubo ethe nkqo isebenzisa inkqubo ye-buffer engapheliyo, apho igumbi eliphezulu liqulethe i-cathode kunye negumbi elisezantsi liqulethe i-anode. Ijeli encinci (engaphantsi kwe-2 mm) igalelwa phakathi kweepleyiti zeglasi ezimbini kwaye ifakwe ukuze umzantsi wejeli ufakwe kwi-buffer kwelinye igumbi kwaye umphezulu ufakwe kwi-buffer kwelinye igumbi. Xa isetyenziswa ngoku, inani elincinci le-buffer lifuduka ngejel ukusuka kwigumbi eliphezulu ukuya kwigumbi elisezantsi.Inkqubo ye-DYCZ - 24DN inokuqhuba iigel ezimbini ngexesha elinye. Ikwagcina isisombululo se-buffer, esinobungakanani obahlukeneyo beepleyiti zeglasi ezichongiweyo, unokwenza iijeli ezahlukeneyo ezishinyeneyo njengemfuno yakho.

Igumbi le-DYCZ-24DN le-electrophoresis linesixhobo sokuphosa i-gel. Sidinga ukudibanisa isixhobo sokuphosa ijeli phambi kovavanyo. Ipleyiti yeglasi ingena ezantsi kwitreyi yokuphosa. Inceda i-gel isilayidi ngaphandle kwe-tray casting xa igqityiwe.Ijeli ibanjwe kwi-tray casting. Inika indawo yokubeka amasuntswana amancinci onqwenela ukuwavavanya. Ijeli iqulethe i-pores evumela ukuba amasuntswana ahambe ngokucothayo ukuya kwicala elichasayo legumbi. Ekuqaleni, i-gel igalelwa kwi-tray njengolwelo olushushu. Njengoko ipholile, nangona kunjalo, ijeli iyaqina.I "comb" ibonakala njengegama layo. Ikama ibekwe kwiindawo zokubeka ecaleni kwetreyi yokuphosa. Ifakwe kwiindawo zokubeka PHAMBI kokuba ijeli eshushu, inyibilike igalelwe. Emva kokuba ijeli iqinile, ikama iyakhutshwa. "Amazinyo" ekama ashiya imingxuma emincinci kwijeli esiyibiza ngokuba "amaqula." Imingxuma yenziwa xa ijeli eshushu nenyibilikayo iqina emazinyweni ekama. Ikama itsalwa emva kokuba ijeli ipholile, ishiya amaqula. Amaqula abonelela ngendawo yokubeka amasuntswana onqwenela ukuwavavanya. Umntu kufuneka alumke kakhulu ukuba angaphazamisi i-gel xa elayisha amaqhekeza. Ukuqhekeka, okanye ukwaphula ijeli kuya kuchaphazela iziphumo zakho.