Isixhobo sokuphosa iGel
Ikati.No.:412-4406
Esi sixhobo se-Gel Casting senzelwe inkqubo ye-DYCZ-24DN.
I-Gel electrophoresis inokuqhutywa nokuba i-horizontal okanye i-vertical orientation. Iijeli ezithe nkqo ngokuqhelekileyo zenziwe nge-matrix ye-acrylamide. Ubungakanani bepore kwezi jeli buxhomekeke kuxinaniso lwamacandelo ekhemikhali: i-agarose gel pores (i-100 ukuya kuma-500 nm ububanzi) zikhulu kwaye zincinci ngokufanayo xa kuthelekiswa ne-acrylamide gelpores (10 ukuya kwi-200 nm ububanzi). Ngokuthelekisa, iimolekyuli ze-DNA kunye ne-RNA zikhulu kunomgca weprotheyini, ezihlala zihlanjululwa ngaphambi, okanye ngexesha lale nkqubo, okwenza kube lula ukuhlalutya. Ngaloo ndlela, iiprotheni ziqhutyelwa kwiigels ze-acrylamide (kwi-vertically) .DYCZ - 24DN i-electrophoresis encinci ye-double vertical esebenzayo kwi-SDS-PAGE kunye ne-native-PAGE. Inomsebenzi wokuphosa iijeli kwindawo yokuqala kunye nesixhobo sethu esikhethekileyo esilungiselelwe ukuphosa ijeli.
