Isixhobo sokuphosa iGel
Ikati. Inombolo: 143-3146
Esi sixhobo sokuphosa ijeli yeyenkqubo ye-DYCP-31DN.
I-Gel electrophoresis inokuqhutywa nokuba i-horizontal okanye i-vertical orientation. Iijeli ezithe tyaba ngokuqhelekileyo zenziwe nge-agarose matrix. Ubungakanani bepore kwezi jeli buxhomekeke kuxinaniso lwamacandelo ekhemikhali: i-agarose gel pores (i-100 ukuya kuma-500 nm ububanzi) zikhulu kwaye zincinci ngokufanayo xa kuthelekiswa ne-acrylamide gelpores (10 ukuya kwi-200 nm ububanzi). Ngokuthelekisa, iimolekyuli ze-DNA kunye ne-RNA zikhulu kunomgca weprotheyini, ezihlala zihlanjululwa ngaphambi, okanye ngexesha lale nkqubo, okwenza kube lula ukuhlalutya. Ngaloo ndlela, i-DNA kunye ne-RNA iamolekyu zihlala ziqhutyelwa kwiigels ze-agarose (ngokuthe tye) .Inkqubo yethu ye-DYCP-31DN yinkqubo ye-electrophoresis ethe tye. Esi sixhobo esibunjiweyo sokuphosa ijeli sinokwenza i-4 yeesayizi ezahlukeneyo zeejeli ngeetreyi zejeli ezahlukeneyo.
