I-DYCP-31DN Comb 13/6 imithombo (1.0mm)

Inkqubo ye-DYCP-31DN yinkqubo ye-electrophoresis ethe tyaba.Kwi-gel electrophoresis ethe tyaba, ijeli iphonswa kwindawo ethe tyaba kwaye ifakwe kwi-buffer esebenzayo ngaphakathi kwebhokisi yegel. Ibhokisi yejeli ihlulwe ibe ngamacandelo amabini, kunye nejeli ye-agarose eyahlula ezimbini. Njengoko bekutshiwo ngaphambili, i-anode ibekwe kwelinye icala, ngelixa i-cathode ikhona kwelinye. I-ionic ebalekayo isithinteli ivumela ukuthambeka kwentlawulo ukuba kuyilwe xa kusetyenziswa umsinga. Ukongeza, i-buffer isebenzela ukupholisa ijeli, etshisa njengentlawulo ifakwe. Isithinteli esisebenzayo sihlala sijikeleziswa kwakhona ukuthintela ukuthambeka kwe-pH ekubeni yenze. Sinobungakanani obahlukeneyo becombs ezisetyenziswayo.Iikhemi ezahlukeneyo zenza le nkqubo ye-electrophoresis ethe tyaba ilungele nasiphi na isicelo sejeli ye-agarose kuquka i-submarine electrophoresis, i-electrophoresis ekhawulezayo kunye neesampuli ezincinci, i-DNA, i-submarine electrophoresis, ukuchonga, ukwahlula nokulungiselela i-DNA, kunye nokulungiselela i-DNA. ukulinganisa ubunzima bemolekyuli.

Ngethuba le-electrophoresis, i-gel yenziwe kwi-tray yokuphosa. Itreyi iqulethe "amaqula" amancinci abambe amasuntswana onqwenela ukuwavavanya. Iimicrolitha ezininzi (µL) zesisombululo esiqulethe amasuntswana onqwenela ukuwavavanya alayishwa ngononophelo equleni. Emva koko, i-buffer, eqhuba umbane wombane, igalelwe kwigumbi le-electrophoresis. Emva koko, i-tray yokuphosa, equkethe iinqununu, ifakwe ngokucophelela kwigumbi kwaye igxininiswe kwi-buffer. Ekugqibeleni, igumbi livaliwe kwaye umthombo wamandla uvuliwe. I-anode kunye ne-cathode, eyenziwe ngumbane wombane, itsala amaqhekeza aphikisanayo. Amasuntswana ahamba kancinane kwijeli ukuya kwelinye icala. Amandla acinyiwe, kwaye i-gel ikhutshwe kwaye ihlolwe.