Inkqubo yokuvala i-DYCP-40C semi-dry blotting isetyenziswa kunye ne-electrophoresis amandla okuhambisa ngokukhawuleza imolekyuli yeprotheyini ukusuka kwijeli ukuya kwimembrane njenge-nitrocellulose membrane. I-Semi-dry blotting yenziwa nge-electrode yepleyiti yegraphite kuqwalaselo oluthe tyaba, isandwiching ijeli kunye nenwebu phakathi kwamaphepha ephepha lokucoca elifakwe isithinteli elisebenza njengedama le-ion. Ngethuba lokudluliselwa kwe-electrophoretic, iimolekyuli ezihlawuliswa kakubi zifuduka ngaphandle kwejel kwaye ziqhubele kwi-electrode efanelekileyo, apho zifakwe kwi-membrane. I-electrodes yepleyiti, ehlulwe kuphela yi-gel kunye ne-stack paper stack, inika amandla aphezulu entsimi (V / cm) kwi-gel, eqhuba ukudluliselwa ngokukhawuleza, ngokukhawuleza.