Yintoni inkqubo yebhayisekile eshushu kwi-PCR?

I-Polymerase Chain Reaction (PCR) bubuchule bebhayoloji yemolekyuli esetyenziselwa ukukhulisa amaqhekeza athile eDNA. Inokuthi ithathwe njengendlela ekhethekileyo yokuphindaphinda kwe-DNA ngaphandle kwento ephilayo. Olona phawu luphambili lwePCR kukukwazi ukonyusa kakhulu amanani omkhondo weDNA.

Isishwankathelo sePolymerase Chain Reaction Cycle

Umfanekiso ovela kwiBioninja

Yintoni inkqubo yebhayisekile eshushu kwi-PCR?

I-PCR inamanyathelo amathathu asisiseko:

1. I-Denaturation ye-Template DNA: I-template ye-DNA ifudunyezwa malunga ne-93 ° C ixesha elithile, ibangela ukuba i-DNA ephindwe kabini ihlukane ibe yimicu enye, ilungiselele ukubopha i-primer kumjikelo wokuphendula olandelayo.

2. I-Annealing ye-Template DNA kunye ne-Primers: Emva kwe-denaturation, iqondo lokushisa liyancipha ukuya kwi-55 ° C, livumela i-primers ukuba ibophe ukulandelelana okuhambelanayo kwi-template ene-stranded DNA.

3. Ukwandiswa kwee-Primers: Kwi-72 ° C, i-DNA template-primer complex iyandiswa ngoncedo lwe-DNA polymerase (njenge-Taq DNA polymerase), isebenzisa i-dNTPs njenge-substrates ukudibanisa i-strand entsha ehambelanayo esekelwe kwi-template ye-DNA.

Beijing LIUYI PCR Machine

Ngokuphinda i-denaturation, i-annealing, kunye neenkqubo zokwandisa, ngakumbi "i-semi-conservative replication strands" iyenziwa, enokuthi isebenze njengeetemplates zemijikelo elandelayo. Umjikelo ngamnye uthatha malunga nemizuzu emi-2 ukuya kwemi-4, ukuvumela ijini ekujoliswe kuyo ukuba yandiswe izihlandlo ezizizigidi kwiiyure ezi-2 ukuya kwezi-3.

I-Beijing Liuyi Biotechnology Co. Ltd (i-Liuyi Biotechnology) ikhethekileyo ekuveliseni izixhobo ze-electrophoresis iminyaka engaphezu kwama-50 kunye neqela lethu lobuchwephesha lobuchwephesha kunye neziko le-R&D. Sinomgca wemveliso othembekileyo nopheleleyo ukusuka kuyilo ukuya ekuhlolweni, kunye nendawo yokugcina impahla, kunye nenkxaso yokuthengisa. Iimveliso zethu eziphambili ziyi-Electrophoresis Cell (itanki / igumbi), i-Electrophoresis Power Supply, i-Blue LED Transilluminator, i-UV Transilluminator, i-Gel Image & Analysis System njl.

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