Electrophoresis Transfer All-in-one System

Inkcazelo emfutshane:

Inkqubo yokudlulisa i-electrophoresis yonke-in-one sisixhobo esenzelwe ukuhambisa iiprotheyini ezihlukeneyo ze-electrophoretically ukusuka kwi-gel ukuya kwi-membrane ukuze kuhlalutywe ngakumbi. Umatshini udibanisa umsebenzi wetanki ye-electrophoresis, ukunikezelwa kwamandla kunye nezixhobo zokudlulisa kwi-system edibeneyo. Isetyenziswa kakhulu kuphando lwebhayoloji yemolekyuli, njengakuhlalutyo lokuchazwa kweprotheyini, ulandelelwano lweDNA, kunye nokubhulwa kweNtshona. Inoncedo lokonga ixesha, ukunciphisa ungcoliseko, kunye nokwenza lula inkqubo yovavanyo.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Inkcazo

Inkcazo yeTanki ye-Electrophoresis
Ubungakanani beGel (LxW)	83×73mm
Ikama	Imithombo eli-10 (Esemgangathweni) Amaqula ali-15 (Ngokuzikhethela)
Ukutyeba kweKamela	1.0 mm (Esemgangathweni) 0.75, 1.5 mm (Ukhetho)
Ipleyiti yeglasi emfutshane	101×73mm
Ipleyiti yeglasi yeSpacer	101×82mm
Buffer Volume	300 ml
Iinkcukacha zeModyuli yokuGqithisela
Indawo yokubhula (LxW)	100×75mm
Inani labaPhathi beGel	2
Umgama we-Electrode	4cm
Buffer Volume	1200ml
Ingcaciso ye-Electrophoresis Power Supply
Ubungakanani (LxWxH)	315 x 290 x 128mm
Umbane ophumayo	6-600V
Imveliso yangoku	4-400mA
Amandla emveliso	240W
Isikhululo semveliso	Izibini ezi-4 ngokuhambelanayo

Inkcazo

Inkqubo yokudlulisa i-electrophoresis yonke-in-one iqulethe itanki ye-electrophoresis ene-lid, ukunikezelwa kwamandla kunye nepaneli yokulawula, kunye nemodyuli yokudlulisa kunye ne-electrodes. I-tank ye-electrophoresis isetyenziselwa ukuphosa kunye nokuqhuba iigels, kwaye imodyuli yokudlulisa isetyenziselwa ukubamba i-gel kunye ne-membrane sandwich ngexesha lenkqubo yokudlulisa, kwaye inebhokisi yokupholisa ukukhusela ukushisa. Ukunikezelwa kwamandla kunika amandla ombane afunekayo ukuqhuba i-gel kwaye uqhube ukuhanjiswa kweeamolekyu ukusuka kwi-gel ukuya kwi-membrane, kwaye inomsebenzi wokulawula ipaneli yokubeka i-electrophoresis kunye neemeko zokudlulisa. Imodyuli yokudlulisa iquka i-electrodes efakwe kwi-tank kwaye idibene ne-gel kunye ne-membrane, ukugqiba isiphaluka sombane esifunekayo ukudluliselwa.

Inkqubo yokudlulisa i-electrophoresis yonke-in-one sisixhobo esibalulekileyo kubaphandi kunye neengcali ezisebenza ngeesampuli zeprotheni. Uyilo lwayo oludityanisiweyo kunye nokulula kokusetyenziswa kuyenza ibe yongezelelo oluxabisekileyo kuyo nayiphi na ilabhu ebandakanyekayo kwibhayoloji yemolekyuli okanye uphando lwebhayoloji.

Isicelo

I-electrophoresis transfer system yonke-in-one sisixhobo esibalulekileyo kwintsimi ye-biology ye-molecular, ngokukodwa kuhlalutyo lweprotheyini. Iiprotheyini ezikhutshelweyo ziye zichongwe kusetyenziswa izilwa-buhlungu ezithile kwinkqubo ebizwa ngokuba yi-Western blotting. Obu buchule buvumela abaphandi ukuba bachonge iiprotheyini ezithile zomdla kwaye balinganise amanqanaba abo okuchaza.

Ibonisiwe

• Imvelisoilingana nesayizi encinci I-PAGE gel electrophoresis;

•Imveliso's parameters, izincedisi zihambelana ngokupheleleyo nemveliso brand eziphambili kwimarike;

•Ubume obukwinqanaba eliphezulu kunye noyilo oluthambileyo;

• Qinisekisa isiphumo esisiso somfuniselo ukusuka ekugalweni kwejeli ukuya kwijeli ebalekayo;

•Ukudlulisa ngokukhawuleza iigels ezincinci;

•Ikhasethi ezimbini zeGel holder zinokufakwa etankini;

•Inokubaleka ukuya kuthi ga kwiijeli ezi-2 ngeyure enye. Inokusebenza ubusuku bonke ukuhanjiswa koxinzelelo oluphantsi;

•Iikhasethi zesibambi sejeli ezinemibala eyahlukeneyo ziqinisekisa ukubekwa kakuhle.

FAQ

Umbuzo: Yintoni i-electrophoresis transfer-in-one system esetyenziselwa yona?

A: Inkqubo yokudlulisa i-electrophoresis yonke-in-one isetyenziselwa ukuhambisa iiprotheni ukusuka kwijeli ye-polyacrylamide kwi-membrane ukuze kuhlalutywe ngakumbi, njengokuchithwa kweNtshona.

Umbuzo: Buthini ubungakanani bejeli enokuthi yenziwe kwaye idluliselwe usebenzisa i-electrophoresis transfer-in-one system?

A: Ukudluliselwa kwe-electrophoresis yonke-in-one inkqubo inokuphosa kwaye iqhube ubungakanani bejel 83X73cm yokuphosa ngesandla, kunye ne-86X68cm yejeli yangaphambili. Indawo yokudluliselwa yi-100X75cm.

Umbuzo: Isebenza njani i-electrophoresis inkqubo yonke-in-one?

A: Inkqubo yokudlulisa i-electrophoresis yonke-in-one isebenzisa i-electrophoresis ukudlulisa iiprotheni ukusuka kwi-gel ukuya kwi-membrane. Iiprotheni ziqala zahlulwa ngobukhulu zisebenzisa i-polyacrylamide gel electrophoresis (PAGE) kwaye emva koko idluliselwe kwi-membrane usebenzisa intsimi yombane.

Umbuzo: Loluphi uhlobo lweembrane ezinokusetyenziswa kunye ne-electrophoresis transfer system yonke-in-one?

A: Iindidi ezahlukeneyo zeembrane zingasetyenziswa kunye ne-electrophoresis transfer system yonke-in-one kuquka i-nitrocellulose kunye ne-PVDF (i-polyvinylidene difluoride) iimbumba.

Umbuzo: Ngaba i-electrophoresis idlulisa inkqubo yonke-kwinye isetyenziselwa uhlalutyo lweDNA?

A: Hayi, i-electrophoresis transfer-in-one system yenzelwe ngokukodwa uhlalutyo lweprotheyini kwaye ayinakusetyenziselwa uhlalutyo lwe-DNA.

Umbuzo: Ziziphi iingenelo zokusebenzisa inkqubo yokudlulisa i-electrophoresis yonke-in-one?

A: Inkqubo yokudlulisa i-electrophoresis yonke-in-one ivumela ukuhanjiswa ngokufanelekileyo kweeprotheyini ukusuka kwi-gel ukuya kwi-membrane, ukubonelela ngovakalelo oluphezulu kunye neenkcukacha ezithile ekuboneni iprotheni. Ikwayinkqubo elungele zonke-kwinye eyenza lula inkqubo yokuvala yaseNtshona.

Umbuzo: I-electrophoresis kufuneka igcinwe njani inkqubo yonke-kwinye?

A: Inkqubo yokudlulisa i-electrophoresis yonke-in-one icocwe emva kokusetyenziswa ngalunye kwaye igcinwe kwindawo ecocekileyo, eyomileyo. Ii-electrodes kunye nezinye iindawo kufuneka zihlolwe rhoqo ukuba kukho nawuphi na umonakalo okanye ukugqoka kunye nokukrazula.